Mouse IgG₁ Isotype Control [Clone HKSP84] — Purified in vivo GOLD™ Functional Grade

The Mouse IgG1 Isotype Control with the clone HKSP84 is used in in vivo mouse studies primarily as a negative control. It is designed to have minimal cross-reactivity with human, mouse, rat, or non-human primate cells, ensuring that any observed effects are due to the specific antibody rather than non-specific binding. Here's how it is typically used:

1. Negative Control for Antibody Treatments: In experiments where a specific antibody is being tested, the HKSP84 clone serves as a control to account for any non-specific effects of injecting an antibody. This helps differentiate between the specific action of the test antibody and any background effects.

2. Assessment of Non-Specific Binding: By using this isotype control, researchers can assess the level of non-specific binding in their experimental model. This is crucial for ensuring that detected signals are genuine and not due to the antibody itself.

3. Validation of Experimental Results: Including the HKSP84 isotype control in experiments helps validate the results by providing a baseline for non-specific interactions. This enhances the credibility of the data by ensuring that any observed effects are due to the specific antibody being tested rather than to background noise.

The HKSP84 clone is formulated in PBS without any carrier proteins or preservatives, making it suitable for in vivo applications where low endotoxin levels are critical to avoid interfering with the experimental outcomes.

Manufactured in an animal-free facility using in vitro cell culture techniques, this isotype control is purified using protein A or G to minimize endotoxin levels and ensure high purity.

Storage and Handling: For short-term storage, it is kept at 2-8°C. For longer periods, it is recommended to aseptically aliquot and freeze at ? -80°C to avoid repeated freeze-thaw cycles.

The correct storage temperature for sterile packaged clone HKSP84 (such as Mouse IgG1 Isotype Control, Clone HKSP84) is 2–8°C (refrigerated) for up to one month in sterile conditions.

For longer-term storage, it is recommended to aseptically aliquot and freeze the antibody, although the specific freezing temperature (typically -20°C or -80°C) should be confirmed with the manufacturer's instructions for the exact product.

Key details:

• Short term (up to 1 month, sterile): 2–8°C
• Long term (beyond 1 month): Aseptically aliquot and freeze (manufacturer guidelines, often -20°C)

Ensure the product remains sterile and avoid repeated freeze-thaw cycles to maintain functional integrity.

Commonly Used Antibodies or Proteins with HKSP84 in the Literature

Current Knowledge from Search Results

The available search results focus primarily on the Mouse IgG1 Isotype Control (Clone HKSP84) as a technical reagent used to control for non-specific binding in immunostaining and flow cytometry experiments, rather than detailing commonly paired antibodies or proteins. The product information emphasizes that HKSP84 is an anti-KLH (keyhole limpet hemocyanin) isotype control antibody, but does not list specific antibodies or proteins it is commonly used alongside.

Broader Context and Typical Experimental Uses

Isotype Controls in Immunology

While HKSP84 is cited as a mouse IgG1 isotype control, isotype controls like HKSP84 are generally used in combination with primary antibodies targeting specific antigens of interest—such as cell surface markers (e.g., CD3, CD4, CD8) or intracellular proteins—to validate the specificity of staining and to rule out nonspecific Fc receptor or secondary antibody binding. For example, in immunophenotyping of immune cells (e.g., T cells, B cells, myeloid cells), researchers use a panel of fluorochrome-conjugated antibodies against targets like CD3, CD4, CD8, CD19, CD11b, etc., each with their own isotype control.

Common Proteins in Antibody Formulations

Although not directly paired with HKSP84, the broader literature on antibody therapeutics and formulations highlights common excipients such as amino acids (e.g., histidine, glycine, proline, arginine, methionine), buffers (e.g., phosphate, acetate), sugars (e.g., sucrose, trehalose), and surfactants (e.g., polysorbate 20, 80) that are used to stabilize antibody solutions. However, these are typically not "paired" in the sense of experimental co-staining but rather co-formulated for stability during storage and administration.

Adjacent Scientific Techniques

In techniques such as ELISA or flow cytometry, HKSP84 would be used alongside enzyme-conjugated secondary antibodies (e.g., anti-mouse IgG-HRP, anti-mouse IgG-AP) and blocking proteins (e.g., bovine serum albumin, BSA; normal serum from the host species) to reduce background staining.

Typical Experimental Pairings

Based on standard immunological practice, HKSP84 would be used in parallel with:

• Primary antibodies specific to the target antigen(s) of interest (exact antibodies depend on the research question—e.g., anti-CD3, anti-CD4, anti-CD8 for T cell subsets).
• Fluorochrome- or enzyme-conjugated secondary antibodies specific to mouse IgG1 for detection.
• Blocking proteins such as BSA or normal serum to minimize nonspecific binding.
• Other isotype controls corresponding to the subclass (IgG2a, IgG2b, etc.) of primary antibodies used in a multiplexed panel.

Summary Table

Conclusion

The specific literature on HKSP84 does not detail a list of commonly paired antibodies or proteins, but standard practice in immunology dictates that it is used alongside primary antibodies (target-dependent), appropriate secondary antibodies, blocking proteins, and sometimes other isotype controls in complex staining panels. For precise pairings, consult the methods section of peer-reviewed papers using HKSP84 in your specific experimental context. If you have a particular experimental system or tissue type in mind, providing those details would allow for a more tailored list of commonly used antibodies.

The search results do not provide specific citations or key findings from the scientific literature regarding clone HKSP84. Clone HKSP84 is associated with a mouse IgG1 isotype control antibody, which is used in immunological research to ensure minimal cross-reactivity with other species' cells and tissues.

Given the lack of specific scientific findings or publications directly referencing clone HKSP84 in the search results, it is necessary to look for broader applications or studies that might involve this antibody. Typically, such antibodies are used as controls in experiments to determine the specificity of primary antibodies. However, without direct citations or studies, we cannot provide detailed findings specifically related to clone HKSP84.

If you are looking for specific research outcomes, it might be helpful to consult scientific databases or literature search platforms for more detailed information.