Mouse IgG2b Isotype Control [Clone MPC-11] — Purified in vivo GOLD™ Functional Grade

Mouse IgG2b Isotype Control [Clone MPC-11] — Purified in vivo GOLD™ Functional Grade

Product No.: I-119

[product_table name="All Top" skus="I-119"]

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Product. No.I-119
Clone
MPC-11
Antibody Type
Isotype Control
Isotype
Mouse
Mouse IgG2b κ

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Antibody Details

Product Details

Host Species
Mouse
Recommended Dilution Buffer
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Working Concentration
This isotype control antibody should be used at the same concentration as the primary antibody.
Applications and Recommended Usage?
Quality Tested by Leinco
FC This isotype control antibody should be used at the same concentration as the primary antibody.
Additional Applications Reported In Literature ?
FA
ICC
ICFC
IF Microscopy
IF Staining
WB
For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
This Mouse IgG2b isotype control antibody has been tested against selected species' cells and tissues to assure minimal cross-reactivity and is a non-reactive isotype-matched control for mouse IgG2b antibodies in most in vivo and in vitro applications.

Leinco Antibody Advisor

Powered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments.

The most common in vivo application of clone MPC-11 in mice is as a mouse IgG2b isotype control antibody. It is specifically used to distinguish non-specific background effects from those caused by a test antibody of the same isotype during in vivo experiments.

Key uses in vivo include:

  • Negative control: MPC-11 does not bind specifically to any known mouse antigen. It acts as a non-reactive, isotype-matched negative control, enabling researchers to identify non-specific binding and background immune responses that are independent of the experimental antibody's specificity.
  • Immunological assays: Frequently used alongside test antibodies in diverse settings such as immuno-oncology, infectious disease, and inflammation studies to ensure valid interpretation of results by ruling out artifacts from general IgG2b interactions.
  • Functional and safety studies: MPC-11 helps confirm that any observed biological effects are due to the specific binding of a targeting antibody, rather than to generic antibody effects or Fc-receptor interactions.
  • Typical in vivo applications: These include evaluation of immune cell depletion, tumor growth studies, and pharmacokinetic or toxicological evaluation of novel antibody therapeutics.

Summary Table: Most Common In Vivo Applications for MPC-11 in Mice

ApplicationPurpose
Negative control (isotype)Distinguish non-specific effects from test antibody effects
Immuno-oncology modelsControl group for efficacy or mechanistic immune studies
Infectious/inflammation modelsValidate response specificity in disease/therapy studies
Pharmacokinetic/toxicologyAttribute observed effects to test antibody, not IgG2b class

Additional context:

  • MPC-11 is formulated for in vivo administration with low endotoxin levels and has been validated to show minimal cross-reactivity and no target specificity in mouse tissues.
  • It is an essential reagent for regulatory and publication standards, ensuring the specificity and reliability of antibody-based therapeutics and mechanistic studies in mouse models.

MPC-11 is not used to target or neutralize antigens in vivo; its primary value is as a non-binding, isotype control to support rigorous, interpretable experiments in immunology and related fields.

While there isn't a comprehensive list of specific antibodies or proteins commonly used with MPC-11 in the provided search results, MPC-11 itself is often used as an isotype control for other antibodies, particularly in studies involving mouse models. It is used to ensure that any observed effects are due to the specific antibody being tested rather than the isotype itself. Here are some contexts and applications where MPC-11 might be used alongside other antibodies:

  1. In Vivo Applications: MPC-11 is often used in conjunction with therapeutic or experimental antibodies to provide a control for specificity in mouse models. This includes studies on immune checkpoint controls (e.g., anti-PD-1/PD-L1/CTLA-4), immune cell depletion controls, and chronic treatment regimens.

  2. Apoptosis Pathways: Although not directly mentioned with MPC-11, antibodies like Caspase-3, Caspase-8, and Caspase-9 are commonly used in apoptosis studies, which might involve using MPC-11 as a control if the experimental antibodies are IgG2b isotype.

  3. Flow Cytometry: MPC-11 is used in flow cytometry setups, often in combination with various dyes or other markers to analyze cell populations. This could involve using it alongside other antibodies for multicolor flow cytometry.

  4. General Research: In broader research contexts, MPC-11 would be used as a control in various experiments involving mouse IgG2b antibodies. The specific antibodies used alongside MPC-11 would depend on the experimental design and the biological question being addressed.

If you're looking for specific antibodies or proteins used with MPC-11 in a particular study or context, it would be helpful to consult the relevant research articles directly.

Key findings from clone MPC-11 citations in scientific literature demonstrate its central role as both a research cell line in immunology and as a widely adopted isotype control antibody in experimental design:

  • Origins and Biology:

    • MPC-11 is a mouse myeloma cell line originally established for studying the structure and synthesis of immunoglobulins, particularly IgG2b subtype antibodies.
    • MPC-11 cells produce fully assembled gamma globulin molecules (IgG2b), as well as immunoglobulin half-molecules, light-chain dimers, and free light chains, resembling the original tumor in these features.
  • Immunoglobulin Class Switching:

    • The MPC-11 cell line was key in early discoveries of immunoglobulin heavy chain class switching. Spontaneous variants of MPC-11 can switch from IgG2b to IgG2a production at high frequency, making this line a powerful model for studying DNA rearrangements associated with antibody class switching.
    • All switch variants express the original heavy chain variable region (VH) gene, so the class switch occurs in the constant region of the immunoglobulin gene rather than the variable region.
    • This system has been critical for understanding the mechanisms and mapping the genetic events underlying class-switch recombination in B cells.
  • Functional Role in Experimental Design:

    • Monoclonal antibodies prepared from MPC-11 are non-reactive isotype-matched controls for mouse IgG2b, used to distinguish specific antibody effects from non-specific Fc-mediated effects or background binding.
      • MPC-11 isotype controls are widely used in:
        • In vivo immunology studies: For example, as controls for immune checkpoint inhibitors (e.g., anti-PD-1/PD-L1)
        • Cell depletion assays: To ensure observed effects are due to the activity of the test antibody
        • Flow cytometry and in vitro assays: To measure and account for non-specific binding by IgG2b antibodies
      • The control is characterized by minimal cross-reactivity and stable biochemical properties.
      • The dosing and application of MPC-11 isotype controls are matched with test antibodies to ensure appropriate experimental comparison (standard dose: 100–250 µg/mouse i.p., with precise amounts and routes adjusted to match the experimental antibody).
      • It is specifically chosen for its lack of functional activity in target engagement, making it ideal as a negative control.
  • Hybrid Resistance and Tumor Models:

    • MPC-11 has also been used in genetic studies on mouse tumor resistance, revealing distinct resistance mechanisms compared to other myeloma cells like P815, controlled by unique genetic loci.

Summary of Impact:

  • MPC-11 is foundational in antibody research: as a model for class switch recombination, as a producer of IgG2b, and most prominently, as the standard isotype control for mouse IgG2b antibodies in immunology, oncology, and cell biology experiments.

If you need findings related to a specific disease model, antibody application, or experimental context using MPC-11, please specify further.

Dosing Regimens of Clone MPC-11 Across Mouse Models

Clone MPC-11 refers to a mouse IgG2b isotype control monoclonal antibody commonly used in immunological research, particularly as a negative control to assess non-specific binding in both in vitro and in vivo assays. Its primary function is not to target or neutralize any specific antigen but to serve as a matched control for experimental antibodies of the same isotype.

General Dosing Principles

  • Dosing regimens for MPC-11 are not standardized across different mouse models; instead, they are specifically matched to the experimental antibody under investigation.
  • Investigators are advised to match the dose, route of administration, and schedule of MPC-11 to those of the primary antibody used in their study, adjusting only if experimental needs require.
  • The working concentration of MPC-11 should generally be the same as that of the primary antibody, ensuring that any observed effects are due to the specificity of the experimental antibody and not non-specific interactions.
  • No typical or universal dosing schedule for MPC-11 is published; the regimen is entirely context-dependent, reflecting the design of the primary experiment.

Variability Across Models

  • Published regimens for MPC-11 vary by context: The dose, frequency, and method of administration are most commonly chosen to mirror the test antibody regimen in each specific model and experiment.
  • There is no evidence in the literature of MPC-11 dosing being adjusted based on strain, age, or disease model. Instead, the emphasis is on consistency with the primary antibody's regimen to maintain experimental validity.
  • Product datasheets and manufacturer guidance do not specify different dosing for different mouse models. They recommend referencing direction on lot-specific datasheets and adjusting only as needed for the experiment.

Practical Recommendations

  • Consult the datasheet for the specific lot of MPC-11 being used, as recommendations may be updated based on manufacturing batches.
  • Determine the optimal working dilution for your application, as optimal concentrations can vary depending on assay type (e.g., flow cytometry, immunohistochemistry, in vivo studies).
  • If in doubt, contact the manufacturer or reference recent literature using MPC-11 in similar experimental setups for guidance on dosing.

Summary Table

AspectStandard Practice for MPC-11 Dosing
DoseMatched to primary antibody
Route & ScheduleMirrors experimental antibody
ConcentrationSame as primary antibody
Model-Specific AdjustmentsNot typically specified; context-dependent

Conclusion

The dosing regimen for clone MPC-11 does not intrinsically vary across different mouse models. Instead, it is tailored to match the specific experimental antibody in use, ensuring that observed effects are attributable to the primary antibody's specificity and not to non-specific interactions. Always align MPC-11 dosing with your experimental antibody’s regimen and consult product-specific guidance for optimal results.

References & Citations

FA
Flow Cytometry
ICC
ICFC
IF Microscopy
IF Staining
in vivo Protocol
Immunoprecipitation Protocol
General Western Blot Protocol

Certificate of Analysis

Formats Available

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Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.