Mouse IgG2b Isotype Control [Clone MPC-11] — Purified in vivo GOLD™ Functional Grade
Antibody DetailsProduct DetailsHost Species Mouse Recommended Dilution Buffer Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C Working Concentration This isotype control antibody should be used at the same concentration as the primary antibody. RRIDAB_2737535 Applications and Recommended Usage? Quality Tested by Leinco FC This isotype control antibody should be used at the same concentration as the primary antibody. Additional Applications Reported In Literature ? FA ICC ICFC IF Microscopy IF Staining WB For specific conjugates of this clone, review literature for suggested application details. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity This Mouse IgG2b isotype control antibody has been tested against selected species' cells and tissues to assure minimal cross-reactivity and is a non-reactive isotype-matched control for mouse IgG2b antibodies in most in vivo and in vitro applications. Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone MPC-11 is used in in vivo mouse studies as a mouse IgG2b isotype control antibody. Its primary function is to serve as a negative control to distinguish non-specific background effects from those caused by a test antibody of the same isotype. Essential context and supporting details:
In summary, MPC-11 acts as a non-reactive, isotype-matched control to ensure that any effects observed in vivo are due to the test antibodys specificity rather than the IgG2b isotype itself or contaminants. Storage Recommendations for Sterile Packaged Clone MPC-11 (Mouse IgG2b, ? Isotype Control)Summary Table
Detailed Guidance
Key Considerations
ConclusionThe correct storage temperature for sterile packaged clone MPC-11 is typically 2–8°C (refrigeration) for most suppliers, with some allowing -20°C for long-term storage if specified by the manufacturer. Do not freeze unless explicitly recommended, and always follow the storage instructions provided with your specific product lot. Based on the research literature, MPC-11 is commonly used alongside several other antibodies and proteins in experimental studies. Here are the key categories and specific examples: Secondary AntibodiesGoat anti-rabbit antibodies are frequently used as secondary detection antibodies in ELISA assays involving MPC-11 cells, particularly those linked to alkaline phosphatase at dilutions of 1:5000. Additionally, secondary antibodies conjugated with horseradish peroxidase are employed in Western blotting applications at 1:5000 dilutions for visualization purposes. Caspase Proteins for Apoptosis StudiesMPC-11 cells are commonly studied alongside several caspase proteins to investigate apoptosis pathways. The primary caspases examined include caspase-3, caspase-8, and caspase-9. These proteins are analyzed through Western blotting to determine their activation status when MPC-11 cells undergo apoptotic processes. Control AntibodiesControl IgG antibodies are routinely used as negative controls in experiments involving MPC-11 cells, typically at concentrations of 200 µg/ml in flow cytometry and apoptosis assays. These serve as important baseline comparisons for specific antibody binding studies. Detection ReagentsSeveral detection and analytical reagents are commonly paired with MPC-11 in experimental protocols:
Comparative Cell LinesIn many studies, MPC-11 is used alongside other myeloma cell lines for comparative analysis, including SP2/0 and NS-1 cells, which are also multiple myeloma cell lines that respond similarly to polyclonal antibodies. Non-myeloma control cell lines such as CT26 (murine colon carcinoma) and B16 (murine melanoma) are also frequently included as negative controls. The MPC-11 clone itself serves as an isotype control antibody targeting KLH (keyhole limpet hemocyanin) and is widely used in both in vivo and in vitro applications as a non-reactive control for mouse IgG2b antibodies. Key findings from scientific literature on MPC-11 clones highlight this mouse myeloma cell line's fundamental role in immunology and cancer research, as well as notable advances in understanding immunoglobulin gene structure, class switching, and its use in therapy modeling. 1. Immunoglobulin Gene Structure and Sequencing:
2. Heavy Chain Class Switching and Genetic Rearrangement:
3. Utility in Cancer Immunotherapy Research:
Additional Context:
These findings collectively establish MPC-11 as a cornerpiece for molecular immunology research and a robust preclinical cancer model. References & CitationsTechnical ProtocolsCertificate of Analysis |
Formats Available
Prod No. | Description |
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I-108 | |
I-130 | |
I-119 | |
I-135 | |
M1415 |
