Polyclonal Human IgG1 Isotype Control – Purified in vivo GOLD™ Functional Grade
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Antibody DetailsProduct DetailsHost Species Human Serum Product Concentration 5.0 - 15 mg/ml Endotoxin Level ≤ 1.0 EU/mg as determined by the LAL method Purity ≥95% by SDS Page Formulation PBS, pH 7.0 - 7.2 containing no stabilizers or preservatives. The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. Product Preparation This functional grade preclinical polyclonal antibody is manufactured in an animal free facility and is purified by a multi-step process including the use of protein A. Pathogen Testing Hepatitis B Surface Antigen: Negative Hepatitis B Core Antigen antibodies: Negative Human Immunodeficiency Virus 1: Negative Human Immunodeficiency Virus 2: Negative Hepatitis C Virus antibodies: Negative * These tests cannot guarantee the absence of infective agents, but we perform the IDEXX h-IMPACT™ 1 test on all material. Country of Origin USA Additional Applications Reported In Literature ? In Vitro Assays: *Isotype Control:Used in Flow Cytometry (FACS), ELISA, and Immunohistochemistry (IHC) to account for non-specific binding to Fc receptors or other cellular components. Blocking Agent: Effective at saturating Fc receptors on cells (such as macrophages or B cells) to prevent background interference during primary antibody staining. In Vivo Studies: Baseline Modeling: Ideal for establishing baseline parameters in animal models receiving humanized monoclonal antibody treatments. Pharmacokinetics: Used as a reference standard for half-life and clearance studies of therapeutic IgG candidates. Assay Development: Acts as a standard for calibrating diagnostic kits and validating antibody specificity. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionBackground This high-purity Polyclonal Human IgG is meticulously isolated from human serum. It serves as an essential negative control (isotype control) to distinguish between specific antibody-antigen interactions and non-specific background signals in both in vivo and in vitro research models. The purification process employs Protein A affinity chromatography, ensuring the removal of non-IgG serum proteins while maintaining primarily a distribution of IgG1 subclass. References & Citations |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
