Recombinant Dengue Virus serotype 2 NS1 Protein

Recombinant Dengue Virus Serotype 2 NS1 Protein (HEK293)

Leinco Technologies offers a highly purified Recombinant Dengue Virus Serotype 2 NS1 protein manufactured in a mammalian expression system to meet the rigorous demands of vaccine development, therapeutics research, and serological diagnostic assay manufacturing.

By utilizing HEK293 cells and state-of-the-art expression techniques, this recombinant DENV2 NS1 protein retains its native post-translational modifications, proper conformational folding, and biologically active hexameric presentation—critical for optimal antigenicity and physiological relevance in dengue pathogenesis studies.

Biological Background & Clinical Relevance: The Global Burden of Dengue Virus (DENV)

Dengue virus is a mosquito-borne flavivirus transmitted primarily by Aedes aegypti and Aedes albopictus. With over one-third of the world’s population living in high-risk tropical and subtropical regions, dengue remains a major global public health challenge, causing an estimated 100 to 400 million infections annually. Infection by any of the four distinct dengue virus serotypes (DENV1–4) can lead to symptoms ranging from mild dengue fever to severe, life-threatening conditions like Dengue Hemorrhagic Fever (DHF) and Dengue Shock Syndrome (DSS).

The Crucial Role of NS1 in Diagnostics and Vaccine Research

Non-Structural Protein 1 (NS1) is a multifunctional glycoprotein essential for viral replication and immune evasion. During an infection, NS1 is secreted by host cells into the bloodstream as a soluble homohexamer. Because high levels of circulating NS1 are present in patient sera during the early acute phase of infection, it serves as the definitive biomarker for early-stage dengue diagnosis.

While the introduction of first-generation dengue vaccines marks a significant milestone, the development of next-generation, pan-serotype vaccines and highly sensitive, type-specific diagnostic assays remains a global priority.

Why Choose a Mammalian (HEK293) Expression System? Many commercially available proteins produced in insect or bacterial systems lack the precise glycosylation patterns found in human infections. Leinco’s HEK293-expressed DENV2 NS1 protein ensures authentic post-translational modifications and a native structural conformation. This mammalian origin eliminates non-specific background interference in human serological assays, providing researchers with superior assay sensitivity, specificity, and reproducible results.

P29990.1

Asp776-Ala1127
1 DSGCVVSWKN KELKCGSGIF ITDNVHTWTE QYKFQPESPS KLASAIQKAH
51 EEGICGIRSV TRLENLMWKQ ITPELNHILS ENEVKLTIMT GDIKGIMQAG
101 KRSLRPQPTE LKYSWKTWGK AKMLSTESHN QTFLIDGPET AECPNTNRAW
151 NSLEVEDYGF GVFTTNIWLK LKEKQDVFCD SKLMSAAIKD NRAVHADMGY
201 WIESALNDTW KIEKASFIEV KNCHWPKSHT LWSNGVLESE MIIPKNLAGP
251 VSQHNYRPGY HTQITGPWHL GKLEMDFDFC DGTTVVVTED CGNRGPSLRT
301 TTASGKLITE WCCRSCTLPP LRYRGEDGCW YGMEIRPLKE KEENLVNSLV
351 TA

Lyophilized Powder

The Dengue Virus Serotype 2 NS1 Recombinant Protein is supplied as a lyophilized powder, prepared from a formulation containing 1X PBS and 5% trehalose.

To maintain the integrity and optimal activity of this recombinant protein, please adhere to the following storage recommendations:

Short-Term Storage: Store at +4°C for up to 1 month.
Long-Term Storage: Store at -80°C for up to 1 year.
Freezing: This product can be frozen. However, avoid multiple freeze-thaw cycles, as repeated temperature shifts can lead to protein denaturation and a loss of activity.

Handling Recommendation: Once fully dissolved, immediately divide the protein solution into single-use working aliquots. The size of the aliquots should match the amount you typically use in a single assay day (e.g., 10 µL to 50 µL per tube).. Use low-binding polypropylene tubes to prevent the protein from sticking to the plastic walls. Snap-freeze (using liquid nitrogen or a dry ice/ethanol bath if available) and store immediately at -80°C for up to 1 year. Once a frozen aliquot is thawed for an experiment, keep it at +4°C and do not refreeze it.

Next Day Blue Ice

1.) Benfrid, S., Park, K., Dellarole, M. et al. Dengue virus NS1 protein conveys pro‐inflammatory signals by docking onto high‐density lipoproteins. EMBO Rep 23, EMBR202153600 (2022). https://doi.org/10.15252/embr.202153600

2.) Smith SA, Zhou Y, Olivarez NP, Broadwater AH, de Silva AM, Crowe JE Jr. Persistence of circulating memory B cell clones with potential for dengue virus disease enhancement for decades following infection. J Virol. 2012 Mar;86(5):2665-75. doi: 10.1128/JVI.06335-11. Epub 2011 Dec 14. PMID: 22171265; PMCID: PMC3302281.