Recombinant Human AOC1 (Amiloride Binding Protein 1)

Amiloride binding protein (ABP1), also referred to as diamine oxidase (DAO) or AOC1, is a ~110 kDa member of the copper-containing amine oxidase family. Naturally occurring as a glycosylated, disulfide-linked homodimer, AOC1 is primarily sequestered within the cytoplasmic granules of epithelial tissues in the kidney, placenta, uterus, lungs, and intestines. The enzyme is released into the extracellular environment upon induction by heparin.

Functionally, AOC1 catalyzes the oxidative deamination of histamine and putrescine, yielding hydrogen peroxide and ammonia. This activity is critical for the catabolism of bioactive polyamines; consequently, AOC1 deficiencies are clinically linked to histaminosis and dietary histamine intolerance.

P19801

EPSPGTLPRK AGVFSDLSNQ ELKAVHSFLW SKKELRLQPS STTTMAKNTV FLIEMLLPKK YHVLRFLDKG ERHPVREARA VIFFGDQEHP NVTEFAVGPL PGPCYMRALS PRPGYQSSWA SRPISTAEYA LLYHTLQEAT KPLHQFFLNT TGFSFQDCHD RCLAFTDVAP RGVASGQRRS WLIIQRYVEG YFLHPTGLEL LVDHGSTDAG HWAVEQVWYN GKFYGSPEEL ARKYADGEVD VVVLEDPLPG GKGHDSTEEP PLFSSHKPRG DFPSPIHVSG PRLVQPHGPR FRLEGNAVLY GGWSFAFRLR SSSGLQVLNV HFGGERIAYE VSVQEAVALY GGHTPAGMQT KYLDVGWGLG SVTHELAPGI DCPETATFLD TFHYYDADDP VHYPRALCLF EMPTGVPLRR HFNSNFKGGF NFYAGLKGQV LVLRTTSTVY NYDYIWDFIF YPNGVMEAKM HATGYVHATF YTPEGLRHGT RLHTHLIGNI HTHLVHYRVD LDVAGTKNSF QTLQMKLENI TNPWSPRHRV VQPTLEQTQY SWERQAAFRF KRKLPKYLLF TSPQENPWGH KRTYRLQIHS MADQVLPPGW QEEQAITWAR YPLAVTKYRE SELCSSSIYH QNDPWHPPVV FEQFLHNNEN IENEDLVAWV TVGFLHIPHS EDIPNTATPG NSVGFLLRPF NFFPEDPSLA SRDTVIVWPR DNGPNYVQRW IPEDRDCSMP PPFSYNGTYR PVHHHHHH

Solution

Produced in HEK cells, this recombinant human AOC1 is a single, glycosylated polypeptide chain consisting of 738 amino acids (residues 20–751). It features a C-terminal 6xHis-tag for purification and has a calculated molecular mass of 84.2 kDa. The protein is isolated using specialized, proprietary chromatographic methods.

This AOC1 protein is supplied in Phosphate-Buffered Saline (pH 7.4) supplemented with 10% Glycerol.

Upon receipt, store the product at -20°C. For immediate use, the solution is stable at 4°C for 2–4 weeks.

- Avoid Freeze-Thaw: Do not subject the protein to multiple freeze-thaw cycles.
- Stabilization: If storing for long periods, supplement with 0.1% HSA or BSA as a carrier protein to enhance stability.

4°C (Blue Ice)