Apolipoprotein E (ApoE) produced by the liver and circulating macrophages, that is a constituent of every plasma lipoprotein except the smallest low density lipoproteins (LDL). It is a key player in the recycling and redistribution of lipids and cholesterol. ApoE is a ligand with a high affinity for low density lipoprotein receptors (LDLR). It regulates the activity of enzymes that metabolize lipids and also makes lipids soluble. Mice and humans that lack ApoE cannot remove excess lipoproteins from the plasma and have an increased risk of atherosclerosis. Defective binding of ApoE to its receptors will lead to accumulation of cholesterol-rich lipoprotein particles in the plasma; this is the cause of type III hyperlipoproteinemia. There are three main isoforms of ApoE all products of alleles at a single gene locus: E2 (Cys112, Cys158), E3 (C112, Arg158), and E4 (Arg112, Arg158). Inheritance of ApoE4 has been associated with earlier onset of Alzheimer’s disease, Parkinson’s disease and increased likelihood of dementia.
>90% by SDS Page and HPLC
<1.0 EU/µg as determined by the LAL method
Protein Accession No.
Amino Acid Sequence
GPKVEQAVET EPEPELRQQT EWQSGQRWEL ALGRFWDYLR WVQTLSEQVQ EELLSSQVTQ ELRALMDETM KELKAYKSEL EEQLTPVAEE TRARLSKELQ AAQARLGADM EDVRGRLVQY RGEVQAMLGQ STEELRVRLA SHLRKLRKRL LRDADDLQKR LAVYQAGARE GAERGLSAIR ERLGPLVEQG RVRAATVGSL AGQPLQERAQ AWGERLRARM EEMGSRTRDR LDEVKEQVAE VRAKLEEQAQ QIRLQAEAFQ ARLKSWFEPL VEDMQRQWAG LVEKVQAAVG TSAAPVPSDN H
State of Matter
Predicted Molecular Mass
The molecular weight of Recombinant Human ApoE4 is Mr 34.4 kDa.
The protein was 0.2 µm filtered and lyophilized from 20 Mm Sodium Phosphate, pH 7.8 + 0.5 mM DTT.
Storage and Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be store at 2-8°C for 1 week. For long-term storage of the reconstituted protein add a carrier protein such as 0.1% BSA, aliquot into working volumes and store at -20°C for up to 3 months in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day Ambient
NCBI Gene Bank
References & Citations
1.) Yang, Hong et al. PLoS One. 2012;7(9):e44430. 2012 Sep 11 PubMed
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.