Recombinant Human Beta-Defensin-3

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Pricing & Details

Product. No.B375
Alternate Names
DEFB-3; BD-3
Expression Host
E. coli
Species
Human
Prod No.
Size
Price
Avail.
Qty
Add to cart
B375-20 μg
20 µg
$224.00
In stock
Max:
Min: 1
Step: 1
B375-100 μg
100 µg
$546.00
In stock
Max:
Min: 1
Step: 1
B375-500 μg
500 µg
$1,949.00
In stock
Max:
Min: 1
Step: 1
B375-1 mg
1 mg
$3,214.00
In stock
Max:
Min: 1
Step: 1

Background

β-Defensin 3, also known as BD3 and DEFB-3, is a membrane-active cationic peptide that functions in inflammation and innate immune responses. There are at least 30 beta-Defensins which are distinguished from alpha-Defensins by the connectivity pattern of their three intramolecular disulfide bonds. The 45 aa mature human BD3 shares 38% and 33% aa sequence identity with mouse and rat BD3, respectively. It shares 18% - 36% aa sequence identity with other human beta-Defensins. BD3 is widely expressed among epithelial tissues, notably by keratinocytes and airway epithelial cells. It is upregulated in response to proinflammatory cytokines, microbial and viral infections, and at the edges of skin wounds. BD3 induction in osteoarthritis chondrocytes promotes MMP1 and 13 production and inhibits TIMP1 and 2 expression. In vivo control of BD3 activity is accomplished in part through cleavage by cathepsins B, L, and S. BD3 displays strain specific microbicidal activity toward a broad spectrum of bacteria and yeast. BD3 also induces monocyte migration, mast cell activation, and a mast cell-dependent increase in vascular permeability. Disruption of the intramolecular disulfide bond pattern in BD3 abrogates its monocyte chemoattractant properties but not its antimicrobial properties.

BD3 inhibits viral infectivity by interacting directly with HIV-1 plus its coreceptor CXCR4, and with HSV glycoprotein B plus its receptor heparan sulfate , and by forming a protective coating on the surface of influenza virus target cells.

Protein Details

Purity
>98% by SDS Page and HPLC
Endotoxin Level
<1.0 EU/µg
Biological Activity
Human Beta-Defensin-3 exhibits antimicrobial activity against gram-positive bacteria S. aureus and gram-negative P. aeruginosa and E. coli.
Expression Host
E. coli
Storage and Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day Ambient
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.