Recombinant Human CD23

CD23, also known as Fc-ε-RII/BLAST-2, is a low affinity IgE Fc receptor which belongs to the C-type lectin structural family and plays a role in the regulation of IgE synthesis and IgE mediated activities (1). It is distinct from the high affinity IgE receptors found on basophils and mast cells, which mediate allergic reactions. CD23 is found both as a transmembrane receptor protein and in a soluble form, which is generated by proteolytic cleavage of membrane bound CD23 (2). It is expressed on most mature, conventional B cells (but not on peritoneal CD5+ B cells), and can also be found on the surface of T cells, macrophages, platelets, eosinophils and EBV transformed B lymphoblasts (3). Expression of CD23 has also been detected in neoplastic cells from cases of B cell chronic Lymphocytic leukemia (4). CD23 is expressed by B cells in the follicular mantle but not by proliferating germinal centre cells.

Histidine Tag

dtt qslkqleera arnvsqvskn leshhgdqma qksqstqisq eleelraeqq rlksqdlels wnlnglqadl ssfksqelne rneasdller lreevtklrm elqvssgfvc ntcpekwinf qrkcyyfgkg tkqwvharya cddmegqlvs ihspeeqdfl tkhashtgsw iglrnldlkg efiwvdgshv dysnwapgep tsrsqgedcv mmrgsgrwnd afcdrklgaw vcdrlatctp pasegsaesm gpdsrpdpdg rlptpsaplh shhhhhhhhh

His

Lyophilized

The predicted molecular weight of Recombinant Human CD23 is Mr 32 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 41 kDa.

32

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Kijimoto-Ochiai, S. et al. (2002) Cell. Mol. Life Sci. 59:648
2. Visan, I. et al. (2003) Euro. J. Immunol. 33:1163
3. Abbas, AK. et al. (2003) Cellular and Molecular Immunology, Saunders
4. Mossalayi, MD. et al. (1991) Annls. New York Acad. Sci. 628:362