Recombinant Human Connective Tissue Growth Factor

CTGF (connective tissue growth factor) is a cysteine-rich, matrix-associated, heparin-binding protein and a member of the CCN family of secreted cysteine rich regulatory proteins and is the major mitogenic and chemoattractant protein produced by umbilical vein and vascular endothelial cells. CTGF stimulates the proliferation and differentiation of chondrocytes, induces angiogenesis, promotes cell adhesion of fibroblasts, endothelial, and epithelial cells, and binds to IGF, TGF beta1, and BMP-4. Cell migration and adhesion are signaled through binding to specific cell surface integrins and to heparin sulfate proteoglycans CTGF, a lower molecular weight isoform containing the C-terminal portion of the full length CTGF protein, exerts full heparin binding, cell adhesion, and mitogenic CTGF activity.

The biological activity of Human Connective Tissue Growth Factor is determined by the dose-dependent stimulation of the proliferation of HUVEC cells. The expected ED₅₀ for this effect is 1.0-2.0 µg/ml.

MGKKCIRTPK ISKPIKFELS GCTSMKTYRA KFCGVCTDGR CCTPHRTTTL PVEFKCPDGE VMKKNMMFIK TCACHYNCPG DNDIFESLYY RKMYGDMA

Lyophilized

The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Next Day Ambient

1. Fine, HA. et al. (2011) J Natl Cancer Inst. 103(15): 1162–1178.PubMed