Recombinant Human EDAR

Recombinant Human EDAR

Product No.: E191

[product_table name="All Top" skus="E191"]

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Prod. No.E191
Expression Host
NS0 Cells

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Background

Ectodysplasin A receptor (EDAR), also known as ED1R, is a cell surface receptor of the tumor necrosis factor receptor (TNFR) family. Defects in EDAR are a cause of hypohidrotic ectodermal dysplasia (HED).1 It is characterized by abnormalities in the hair, teeth and sweat glands. EDAR is also the genetic determinant of hair fiber thickness variation among Asian populations.2 EDA has two splice variants, EDA-A1 and EDA-A2, which differ by only two amino acids. Despite this minor difference, EDAR only binds to EDA-A1, whereas EDA-A2 binds to XEDAR. It mediates the activation of NF-κB and JNK and may promote caspase independent cell death.3

Protein Details

Purity
>90% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Fusion Protein Tag
Fc Fusion Protein
Protein Accession No.
Amino Acid Sequence
eysn cgeneyynqt tglcqecppc gpgeepylsc gygtkdedyg cvpcpaekfs kggyqicrrh kdcegffrat vltpgdmend aecgpclpgy ymlenrprni ygmvcyscll appntkecvg atsgasanfp gtsgsstlsp fqhahkelsg qghlatalid iegrmdpksc dkthtcppcp apellggpsv flfppkpkdt lmisrtpevt cvvvdvshed pevkfnwyvd gvevhnaktk preeqynsty rvvsvltvlh qdwlngkeyk ckvsnkalpa piektiskak gqprepqvyt lppsrdeltk nqvsltclvk gfypsdiave wesngqpenn ykttppvlds dgsfflyskl tvdksrwqqg nvfscsvmhe alhnhytqks lslspgk
N-terminal Sequence Analysis
Glu27
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human EDAR is Mr 44.3 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 55-60 kDa.
Predicted Molecular Mass
44.3
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Headon, DJ. et al. (2008) Hum. Genet. 29:1405
2. Tokunaga, K. et al. (2008) Hum. Genet. 124:179
3. Kumar, A. et al. (2001) J. Biol. Chem. 276:2668
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.