Recombinant Human Exodus-2 (CCL21)

Home/Recombinant Proteins/Recombinant Human Exodus-2 (CCL21)

Pricing & Details

Product. No.E113
Alternate Names
Chemokine (C-C Motif) Ligand 21, 6Ckine, CKb9, ECL, MGC34555, SCYA21, SLC, TCA4, Exodus-2
Expression Host
E. coli
Species
Human
Prod No.
Size
Price
Avail.
Qty
Add to cart
E113-25 µg
25 µg
$349.00
In stock
Max:
Min: 1
Step: 1

Background

Chemokine (C-C motif) ligand 21 (CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine (because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine (SLC). CCL21 elicits its effects by binding to a cell surface chemokine receptor known as CCR7.

In the cancer biology CCL21 seems to have multifaceted roles. CCL21 attracts CCR7 bearing cells especially T and dendritic cells but also various cancer cells. Besides the antitumour role as leukocyte recruiting, CCL21 has been shown to facilitate dendritic cell functions and to exert an angiostatic effect.

Human and mouse 6Ckine are highly conserved, exhibiting 86% amino acid sequence identity.

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human Exodus-2 was determined by its ability to chemoattract 5 - 10 day cultured human lymphocytes and BaF/3 cellstransfected with hCCR-7. The expected ED50 for these effects are typically 0.2 - 0.6 μg/ml and 3 - 15 ng/ml, respectively.
Expression Host
E. coli
Protein Accession No.
Q6ICR7
Amino Acid Sequence
sdggaqd cclkysqrki pakvvrsyrk qepslgcsip ailflprkrs qaelcadpke lwvqqlmqhl dktpspqkpa qgcrkdrgas ktgkkgkgsk gckrtersqt pkgp
N-terminal Sequence Analysis
Ser24
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human CCL21 is Mr 12 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 16-17 kDa (reducing conditions).
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Prod No.
Description
E113
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Leinco Technologies uses cookies to improve your experience and our website service. By continuing to browse our website, you accept our cookie policy. Ok