Recombinant Human Exodus-2 (CCL21)

Chemokine (C-C motif) ligand 21 (CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine (because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine (SLC). CCL21 elicits its effects by binding to a cell surface chemokine receptor known as CCR7.

In the cancer biology CCL21 seems to have multifaceted roles. CCL21 attracts CCR7 bearing cells especially T and dendritic cells but also various cancer cells. Besides the antitumour role as leukocyte recruiting, CCL21 has been shown to facilitate dendritic cell functions and to exert an angiostatic effect.

Human and mouse 6Ckine are highly conserved, exhibiting 86% amino acid sequence identity.

The biological activity of Human Exodus-2 was determined by its ability to chemoattract 5 - 10 day cultured human lymphocytes and BaF/3 cellstransfected with hCCR-7. The expected ED₅₀ for these effects are typically 0.2 - 0.6 μg/ml and 3 - 15 ng/ml, respectively.

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Ser24

Lyophilized

The predicted molecular weight of Recombinant Human CCL21 is Mr 12 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 16-17 kDa (reducing conditions).

12

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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