Recombinant Human Fas

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Pricing & Details

Product. No.F158
Alternate Names
TNFRSF6, CD95, Apo-1, Fas Antigen
Expression Host
NS0 cells
Species
Human
Prod No.
Size
Price
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F158-50 µg
50 µg
$369.00
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Background

Fas and Fas Ligand (FasL) belong to the TNF superfamily and are type I and type II transmembrane proteins, respectively. Binding of FasL to Fas triggers apoptosis in Fas-bearing cells. The mechanism of apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. sFasR is capable of inhibiting FasL-induced apoptosis by acting as a decoy receptor that serves as a sink for FasL.

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human Soluble Fas Receptor is determined by its ability to inhibit the cytotoxicity of Jurkat cells. The expected ED50 for this effect is 10-15 mg/ml in the presence of 2 ng/ml of human FasL.
Fusion Protein Tag
Fc Fusion Protein
Expression Host
NS0 cells
Protein Accession No.
Q5T9P3
Amino Acid Sequence
rlss ksvnaqvtdi nskglelrkt vttvetqnle glhhdgqfch kpcppgerka rdctvngdep dcvpcqegke ytdkahfssk crrcrlcdeg hgleveinct rtqntkcrck pnffcnstvc ehcdpctkce hgiikectlt sntkckeegs rsniegrmdp kscdkthtcp pcpapellgg psvflfppkp kdtlmisrtp evtcvvvdvs hedpevkfnw yvdgvevhna ktkpreeqyn styrvvsvlt vlhqdwlngk eykckvsnka lpapiektis kakgqprepq vytlppsrde ltknqvsltc lvkgfypsdi avewesngqp ennykttppv ldsdgsffly skltvdksrw qqgnvfscsv mhealhnhyt qkslslspgk hhhhhh
N-terminal Sequence Analysis
Arg17 predicted
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human Fas is Mr 45 kDa.
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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