Recombinant Human FGF-6

Fibroblast growth factor 6 (FGF6), also known as hst-2 or hst-1-related, is a distinct member of the fibroblast growth factor (FGF) family, which is widely recognized for its roles in cell growth, survival, and diverse biological processes such as embryonic development, tissue repair, and tumor progression. Uniquely, FGF6 expression is predominantly limited to the muscle cell lineage during both development and in adult skeletal muscles, indicating its specialized role in muscle biology. It plays a critical dual role in muscle regeneration by promoting the proliferation and migration of myoblasts as well as enhancing muscle differentiation and hypertrophy in a dose-dependent manner. This key function is linked to its ability to activate satellite cells and regulate myogenic factors, making FGF6 vital for effective muscle repair and growth after injury. This muscle-specific expression and function highlight FGF6 as an essential factor in myogenesis and skeletal muscle regeneration.

P10767.4

MGTRANNTLL DSRGWGTLLS RSRAGLAGEI AGVNWESGYL VGIKRQRRLY CNVGIGFHLQ VLPDGRISGT HEENPYSLLE ISTVERGVVS LFGVRSALFV AMNSKGRLYA TPSFQEECKF RETLLPNNYN AYESDLYQGT YIALSKYGRV KRGSKVSPIM TVTHFLPRI.

Lyophilized

Recombinant human FGF6 produced in E. coli consists of a single, non-glycosylated polypeptide chain composed of 169 amino acids, with a molecular weight of approximately 18.9 kDa. The protein is purified using proprietary chromatographic methods to ensure high purity and quality suitable for research applications.

For reconstitution, it is advised to use sterile high-purity water (18MΩ-cm) with the lyophilized protein at a concentration of at least 100µg/ml. Subsequent dilutions into other aqueous solutions can be performed after this initial step.

The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Next Day Ambient

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4. Chanoine C. et al. (2006) Biochim Biophys Acta 1763: 773
5. Floss T, Arnold HH, Braun T. A role for FGF-6 in skeletal muscle regeneration. Genes Dev. 1997 Aug 15;11(16):2040-51. doi: 10.1101/gad.11.16.2040. PMID: 9284044; PMCID: PMC316448.