Recombinant Human FGF-Basic

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Pricing & Details

Product. No.F110
Alternate Names
Fibroblast Growth Factor-Basic, B-FGF, FGF-2, FGF-β, FGFB, Prostatropin, NUDT6
Expression Host
E. coli
Species
Human
Prod No.
Size
Price
Avail.
Qty
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F110-25 µg
25 µg
$259.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Data

Background

Basic fibroblast growth factor (bFGF), also known as FGF-2 and FGF-β, is a non-glycosylated heparin binding growth factor and member of the FGF family of mitogenic proteins. Members of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation (1). bFGF is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland, liver, placenta, and monocytes, epithelial and endothelial cells. It is secreted by mechanisms other than the classical protein secretion pathway due to the lack of a signal peptide. Acidic FGF (aFGF) and bFGF bind to the same high affinity receptors (2). Binding of bFGF to heparin or cell surface heparan sulfate proteoglycans is a prerequisite for ligation of bFGF to these receptors. aFGF and BFGF have a similar range of biological activities which are implicated in several important physiological and pathological processes, such as embryonic development and differentiation, morphogenesis, angiogenesis, and wound healing (3-4).

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human FGF-basic was monitored in a mitogenic assay by measuring the FGF basic dependent 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts (Rizzino, A. et al., 1988. Cancer Research 48:4266 - 4271). The expected ED50 for this effect is typically 0.1 - 0.25 ng/ml.
Expression Host
E. coli
Protein Accession No.
NP_001997
Amino Acid Sequence
gtmaagsit tlpalpedgg sgafppghfk dpkrlyckng gfflrihpdg rvdgvreksd phiklqlqae ergvvsikgv canrylamke dgrllaskcv tdecffferl esnnyntyrs rkytswyval krtgqyklgs ktgpgqkail flpmsaks
N-terminal Sequence Analysis
Gly132
State of Matter
Solution
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human FGF-Basic is Mr 17.4 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is 19 kDa (reducing conditions).
Formulation
This recombinant protein solution was 0.2 µm filtered and formulated in Tris and NaCl.
Storage and Stability
This protein is stable for > twelve months when stored at -20°C to -70°C. After thawing and addition of a carrier protein, this protein may be stored at 2°C to 8°C for one month or for up to three months at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day 2-8°C
PubMed
NCBI Gene Bank

References & Citations

1. Swain, JL. et al. (1991) Developement 111: 741
2. Grevers, G. et al. (1997) Laryngorhinootologie 76: 421
3. Bühring, HJ. et al. (2007) Differentiation. 75(4):279-91
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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