Purified Recombinant Human FGF-Basic (>98%)
This purified monoclonal antibody is aseptically filtered and lyophilized from PBS with no carrier proteins or preservatives added.
Storage and Handling
This lyophilized monoclonal antibody should be stored desiccated at -20°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
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Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is at a concentration of approximately 0.5-1.0 µg/ml. A suggested standard for this assay is PN:F110.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Mouse Anti-Human Fibroblast Growth Factor-basic (FGF-basic) recognizes Human FGF-basic. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Acidic and basic fibroblast growth factors (FGFs) are members of a family of proteins that exert pleiotropic effects in a range of cell types including skeletal myocytes.1 Fibroblast growth factor basic (FGF basic) significantly promote angiogenesis; a central event for the growth and metastasis of solid tumors.2 It is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland, liver, placenta, and monocytes, epithelial and endothelial cells. FGF-basic plays a central role during prenatal development and postnatal growth and regeneration of tissues, by promoting cellular proliferation and differentiation. FGF-basic is one of the angiogenesis factors and may promote wound healing.3
References & Citations
1. Swain, JL. et al. (1991) Developement 111: 741
2. Grevers, G. et al. (1997) Laryngorhinootologie 76: 421
3. Bühring, HJ. et al. (2007) Differentiation. 75(4):279-91
Products are for research use only. Not for use in diagnostic or therapeutic procedures.