Recombinant Human FGF R2α (IIIc)
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Protein DetailsPurity >90% by SDS-PAGE and analyzed by silver stain. Endotoxin Level <0.1 EU/µg as determined by the LAL method Biological Activity The biological activity of Human FGF R2α (IIIc) was determined by its ability to inhibit FGF acidic-dependent proliferation of NR6R-3T3 mouse fibroblasts. The expected ED<sub>50</sub> for this effect is typically 0.5 - 2 ng/ml. Fusion Protein Tag Fc Fusion Protein Protein Accession No. Amino Acid Sequence rpsfslved ttlepeeppt kyqisqpevy vaapgeslev rcllkdaavi swtkdgvhlg pnnrtvlige ylqikgatpr dsglyactas rtvdsetwyf mvnvtdaiss gddeddtdga edfvsensnn krapywtnte kmekrlhavp aantvkfrcp aggnpmptmr wlkngkefkq ehriggykvr nqhwslimes vvpsdkgnyt cvveneygsi nhtyhldvve rsphrpilqa glpanastvv ggdvefvckv ysdaqphiqw ikhvekngsk ygpdglpylk vlkaagvntt dkeievlyir nvtfedagey tclagnsigi sfhsawltvl papgrekeit aspdyle (Human FGF R2α IIIc Leu27-Glu377) ieg rmdpkscdkt htcppcpape llggpsvflf ppkpkdtlmi srtpevtcvv vdvshedpev kfnwyvdgve vhnaktkpre eqynstyrvv svltvlhqdw lngkeykckv snkalpapie ktiskakgqp repqvytlpp srdeltknqv sltclvkgfy psdiavewes ngqpennykt tppvldsdgs fflyskltvd ksrwqqgnvf scsvmhealh nhytqkslsl spgk N-terminal Sequence Analysis Arg22 & Leu27 State of Matter Lyophilized Predicted Molecular Mass The predicted molecular weight of Recombinant Human FGF R2α (IIIc) is Mr 66 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 100-110 kDa. Predicted Molecular Mass 66 Formulation This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives. Storage and Stability This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. Country of Origin USA Shipping Next Day Ambient NCBI Gene Bank Technical ProtocolsCertificate of AnalysisIMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein. |
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