Recombinant Human HGF

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Pricing & Details

Product. No.H255
Alternate Names
Hepatocyte Growth Factor, Scatter Factor [SF], Hepatopoietin A [HPTA], F-TCF, HGFB
Expression Host
Sf 21 Insect Cells
Species
Human
Applications
ELISA
Prod No.
Size
Price
Avail.
Qty
Add to cart
H255-5 µg
5 µg
$115.00
In stock
Max:
Min: 1
Step: 1
H255-25 µg
25 µg
$1,095.00
In stock
Max:
Min: 1
Step: 1
H255-250 µg
250 µg
$2,500.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Background

Hepatocyte growth factor/scatter factor (HGF/SF) is a paracrine cellular growth, motility and morphogenic factor. It is secreted by mesenchymal cells and targets and acts primarily upon epithelial cells and endothelial cells, but also acts on haemopoietic progenitor cells. It has been shown to have a major role in embryonic organ development, in adult organ regeneration and in wound healing.1

Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration.

Protein Details

Purity
>95% by SDS-PAGE and analyzed by silver stain.
Biological Activity
The biological activity of Human HGF was determined by measuring its ability to stimulate 3H-thymidine incorporation in the HGF responsive monkey epithelial cell line, 4MBr-5 (Rubin, J.S. et al., 1991, Proc. Natl. Acad. Sci. 88:415 - 419). The expected ED50 for this effect is typically 20.0 - 40.0 ng/ml.
Expression Host
Sf 21 Insect Cells
Protein Accession No.
Amino Acid Sequence
qrkrrntih efkksakttl ikidpalkik tkkvntadqc anrctrnkgl pftckafvfd karkqclwfp fnsmssgvkk efghefdlye nkdyirncii gkgrsykgtv sitksgikcq pwssmipheh sflpssyrgk dlqenycrnp rgeeggpwcf tsnpevryev cdipqcseve cmtcngesyr glmdhtesgk icqrwdhqtp hrhkflpery pdkgfddnyc rnpdgqprpw cytldphtrw eycaiktcad ntmndtdvpl etteciqgqg egyrgtvnti wngipcqrwd sqyphehdmt penfkckdlr enycrnpdgs espwcfttdp nirvgycsqi pncdmshgqd cyrgngknym gnlsqtrsgl tcsmwdknme dlhrhifwep dasklnenyc rnpdddahgp wcytgnplip wdycpisrce gdttptivnl dhpviscakt kqlrvvngip trtnigwmvs lryrnkhicg gslikeswvl tarqcfpsrd lkdyeawlgi hdvhgrgdek ckqvlnvsql vygpegsdlv lmklarpavl ddfvstidlp nygctipekt scsvygwgyt glinydgllr vahlyimgne kcsqhhrgkv tlneseicag aekigsgpce gdyggplvce qhkmrmvlgv ivpgrgcaip nrpgifvrva yyakwihkii ltykvpqs
N-terminal Sequence Analysis
alpha chain: No results obtained, Gln32 predicted; beta chain: Val495
State of Matter
Lyophilized
Predicted Molecular Mass
This protein consists of a disulfide-linked heterodimer and an unprocessed single chain. The predicted molecular mass is 53.7 kDa (α chain), and 26 kDa (β chain). The actual molecular mass of Recombinant Human HGF is Mr 60-70 kDa under nonreducing conditions; and 60 kDa and 33-34 kDa under reducing conditions.
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in sodium phosphate (NaH2PO4) and sodium chloride (NaCl).
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank

References & Citations

1. Lyon, M. et al. (2000) Proteoglycans: structure, biology and molecular interactions.: 27
Elisa Sandwich Protocol
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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