Anti-Human HGF

Product No.: H1484

[product_table name="All Top" skus="H1484"]

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Clone
24612
Target
HGF
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Hepatocyte Growth Factor, Scatter Factor [SF], Hepatopoietin A [HPTA], F-TCF, HGFB
Isotype
IgG1
Applications
IHC
,
N
,
WB

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human HGF (Accession # P14210)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human HGF this monoclonal antibody can be used at a concentration of 1 - 2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T263. The detection limit for Human HGF is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:H255.
Additional Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human HGF bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human HGF (at 100 ng/ml) is 0.1 - 0.3 µg/ml.
Immunohistochemistry: Suitable for use at concentration of 8-25 µg/mL.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human Hepatocyte Growth Factor (HGF) (Clone 24612) recognizes an epitope on Human HGF. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Hepatocyte growth factor/scatter factor (HGF/SF) is a paracrine cellular growth, motility and morphogenic factor. It is secreted by mesenchymal cells and targets and acts primarily upon epithelial cells and endothelial cells, but also acts on haemopoietic progenitor cells. It has been shown to have a major role in embryonic organ development, in adult organ regeneration and in wound healing.1

Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration.

Antigen Details

PubMed
Research Area
Other Molecules

References & Citations

1. Lyon, M. et al. (2000) Proteoglycans: structure, biology and molecular interactions.: 27
IHC
N
General Western Blot Protocol

Certificate of Analysis

Formats Available

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Prod No.
Description
H1484
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.