Recombinant Human IFNγ

Interferon-gamma (IFN-γ) or type II interferon is a dimerized soluble cytokine that is the only member of the type II class of interferons.¹ It is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.² IFN-γ has antiviral, immunoregulatory, and anti-tumour properties.³

The biological activity of Human IFN-Gamma was measured in anti-viral assays using HeLa cells infected with EMC virus (Meager, A., 1987, Lymphokines and Interferons, a Practical Approach, Clemens, M.J., Morris, A.G. and Gearing, A.J.H. eds., IRL Press, p. 129). The expected ED₅₀ for this effect is typically 0.3 - 1.5 ng/ml.

CAA31639

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Solution

The predicted molecular weight of Recombinant Human IFN-γ is Mr 16.9 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is 17 kDa (reducing conditions).

This recombinant protein solution was 0.2 µm filtered and formulated in modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.

For short term storage, this protein can be stored at 2-8°C for 1 month after reconstitution. For long term storage, store at -20 to -70°C as supplied for 12 months or reconstituted for 3 months in a manual defrost freezer. Avoid repeat freeze-thaw cycles.

Polar Packs

1. Goeddel, DV. et al. (1982) Nature 298: 859
2. Wilson, CB. et al. (2007) Adv. Immunol. 96: 41
3. Hume, DA. et al. (2004) J Leukoc Biol. 75: 163