Recombinant Human IFNγ

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Pricing & Details

Product. No.I-179
Alternate Names
Interferon Gamma, Immune Interferon, Type II Interferon, T Cell Interferon, MAF, IFNG, IFG, IFI
Expression Host
E. coli
Prod No.
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I-179-100 µg
100 µg
In stock
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.



Interferon-gamma (IFN-γ) or type II interferon is a dimerized soluble cytokine that is the only member of the type II class of interferons.1 It is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.2 IFN-γ has antiviral, immunoregulatory, and anti-tumour properties.3

Protein Details

>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.01 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human IFN-Gamma was measured in anti-viral assays using HeLa cells infected with EMC virus (Meager, A., 1987, Lymphokines and Interferons, a Practical Approach, Clemens, M.J., Morris, A.G. and Gearing, A.J.H. eds., IRL Press, p. 129). The expected ED50 for this effect is typically 0.3 - 1.5 ng/ml.
Expression Host
E. coli
Protein Accession No.
Amino Acid Sequence
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N-terminal Sequence Analysis
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human IFN-γ is Mr 16.9 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is 17 kDa (reducing conditions).
This recombinant protein solution was 0.2 µm filtered and formulated in modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.
Storage and Stability
For short term storage, this protein can be stored at 2-8°C for 1 month after reconstitution. For long term storage, store at -20 to -70°C as supplied for 12 months or reconstituted for 3 months in a manual defrost freezer. Avoid repeat freeze-thaw cycles.
Country of Origin
Polar Packs

References & Citations

1. Goeddel, DV. et al. (1982) Nature 298: 859
2. Wilson, CB. et al. (2007) Adv. Immunol. 96: 41
3. Hume, DA. et al. (2004) J Leukoc Biol. 75: 163
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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