Anti-Rat IFNγ (Clone 88915)

Anti-Rat IFNγ (Clone 88915)

Product No.: I-683

[product_table name="All Top" skus="I-683"]

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Clone
88915
Target
IFN gamma
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Immune Interferon, Type II Interferon, T Cell Interferon, MAF, IFNG, IFG, IFI, Interferon Gamma
Isotype
IgG1
Applications
N
,
WB

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Antibody Details

Product Details

Reactive Species
Rat
Host Species
Mouse
Immunogen
E. coli-Derived Recombinant Rat Interferon-γ
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Rat IFN-γ this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Rat IFN-γ is 10 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Additional Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Rat IFN-γ bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Rat IFN-γ (at 2.5 ng/ml) is 0.5 - 1.5 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Mouse Anti-Rat Interferon Gamma (IFN-γ) (Clone 88915) recognizes an epitope on Rat IFN-γ. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows less than 1% cross-reactivity with rmIFN-γ and no cross-reactivity rhIFN-γ.
Background
Interferon-gamma (IFN-γ) or type II interferon is a dimerized soluble cytokine that is the only member of the type II class of interferons.1 It is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.2 IFN-γ has antiviral, immunoregulatory, and anti-tumour properties.3
PubMed
NCBI Gene Bank ID
Research Area
Other Molecules

References & Citations

1. Goeddel, DV. et al. (1982) Nature 298: 859
2. Wilson, CB. et al. (2007) Adv. Immunol. 96: 41
3. Hume, DA. et al. (2004) J Leukoc Biol. 75: 163
N
General Western Blot Protocol

Certificate of Analysis

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Formats Available

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Prod No.
Description
I-683
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.