Antibody DetailsProduct DetailsReactivity Species Mouse Host Species Goat Immunogen NS0-Derived Mouse IFN-γ R1 Extracellular Domain Endotoxin Level <0.1 EU/µg as determined by the LAL method Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830723 Applications and Recommended Usage? Quality Tested by Leinco ELISA Indirect This antibody can be used in an indirect detection ELISA at 0.5-1.0 µg/ml with a suitable second step reagent such as PN:G505. The detection sensitivity of this indirect ELISA for Mouse IFN-γ RI is approximately 0.2 ng/well. WB To detect Mouse IFN-γ RI this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Mouse IFN-γ RI is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Other Applications Reported In Literature ? N This antibody is useful for neutralization of Mouse IFN-γ RI bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse IFN-γ RI (at 1.0 ng/ml) is 0.2-1.0 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Goat Anti-Mouse IFN-γ RI recognizes Mouse IFN-γ RI. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Antigen Distribution The IFN-γ receptor is expressed at moderate levels on virtually every cell with the exception of erythrocytes. Background IFNγ Rα, or IFNγ R1, is a 90-100 kD type I transmembrane protein that is structurally related to IL-10 receptor. IFNγ Receptor consists of α and ß chains and requires association of JAK1, JAK2 and Stat1 for IFN-γ signal transduction which induces tyrosine phosphorylation of IFN-γ Rα leading to the formation of a docking site on the activated receptor for Stat1, which specifically activates IFN-γ induced gene transcription. Antigen DetailsProtein PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Calderon, J. et al. (1988) PNAS 85:4837 2. Garcia-Sastre, A. et al. (2002) Int. Rev. Immunol. 21:305 3. Fish, E. et al. (2002) J. Interferon Cytokine Res. 22:835 Technical Protocols  |
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