Anti-Human CD119 (IFN-γ Rα Chain)

Anti-Human CD119 (IFN-γ Rα Chain)

Product No.: I-600

[product_table name="All Top" skus="I-600"]

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Target
IFNγ Rα
Product Type
Polyclonal Antibody
Alternate Names
Interferon Gamma Receptor 1, CD119, IFN-γR, IFN-γRα, IFN-α R1, CDw119
Applications
ELISA Indirect
,
N
,
WB

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Select Product Size
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Goat
Immunogen
NS0-Derived Mouse IFN-γ R1 Extracellular Domain
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
State of Matter
Lyophilized
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Indirect This antibody can be used in an indirect detection ELISA at 0.5-1.0 µg/ml with a suitable second step reagent such as PN:G505. The detection sensitivity of this indirect ELISA for Mouse IFN-γ RI is approximately 0.2 ng/well.
WB To detect Mouse IFN-γ RI this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Mouse IFN-γ RI is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Additional Applications Reported In Literature ?
N This antibody is useful for neutralization of Mouse IFN-γ RI bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse IFN-γ RI (at 1.0 ng/ml) is 0.2-1.0 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Goat Anti-Mouse IFN-γ RI recognizes Mouse IFN-γ RI. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
IFNγ Rα, or IFNγ R1, is a 90-100 kD type I transmembrane protein that is structurally related to IL-10 receptor. IFNγ Receptor consists of α and ß chains and requires association of JAK1, JAK2 and Stat1 for IFN-γ signal transduction which induces tyrosine phosphorylation of IFN-γ Rα leading to the formation of a docking site on the activated receptor for Stat1, which specifically activates IFN-γ induced gene transcription.
Antigen Distribution
The IFN-γ receptor is expressed at moderate levels on virtually every cell with the exception of erythrocytes.
PubMed
NCBI Gene Bank ID

References & Citations

1. Calderon, J. et al. (1988) PNAS 85:4837
2. Garcia-Sastre, A. et al. (2002) Int. Rev. Immunol. 21:305
3. Fish, E. et al. (2002) J. Interferon Cytokine Res. 22:835
Indirect Elisa Protocol
N
General Western Blot Protocol

Certificate of Analysis

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Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.