Insulin-like growth factor 2 (IGF-2) is a polypeptide hormone with structural and functional homology with IGF-I and pro-insulin.1 IGF-2 exerts its effects by binding to the IGF-1 receptor. IGF2 may also bind to the IGF-2 receptor. The major role of IGF2 is growth promoting hormone during gestation. IGF-2 has been shown to be an important regulator of fetoplacental growth.2
>97% by SDS-PAGE and analyzed by silver stain.
<0.1 EU/µg as determined by the LAL method
The biological activity of Human IGF-II was determined by a serum-free cell proliferation assay using the human cell line MCF-7 (Karey, K.P. et al., Cancer Research, 1988, 48:4083). The expected ED50 for this effect is typically 1.5 – 6 ng/ml.
Protein Accession No.
Amino Acid Sequence
ayrpset lcggelvdtl qfvcgdrgfy fsrpasrvsr rsrgiveecc frscdlalle tycatpakse
N-terminal Sequence Analysis
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human IGF-II is Mr 7.5 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is 7 kDa (reducing conditions).
This recombinant protein was 0.2 µm filtered and lyophilized from a sterile solution of 0.1M acetic acid.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
Next Day Ambient
NCBI Gene Bank
References & Citations
1. Hoeflich, A. et al. (1994) Hormone Research 41: 66
2. Gruslin, A. et al. (2005) The National Acad. of Sci.
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.