Recombinant Human IGF-II

Insulin-like growth factor 2 (IGF-2) is a polypeptide hormone with structural and functional homology with IGF-I and pro-insulin.¹ IGF-2 exerts its effects by binding to the IGF-1 receptor. IGF2 may also bind to the IGF-2 receptor. The major role of IGF2 is growth promoting hormone during gestation. IGF-2 has been shown to be an important regulator of fetoplacental growth.²

The biological activity of Human IGF-II was determined by a serum-free cell proliferation assay using the human cell line MCF-7 (Karey, K.P. et al., Cancer Research, 1988, 48:4083). The expected ED₅₀ for this effect is typically 1.5 – 6 ng/ml.

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Ala25

Lyophilized

The predicted molecular weight of Recombinant Human IGF-II is Mr 7.5 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is 7 kDa (reducing conditions).

This recombinant protein was 0.2 µm filtered and lyophilized from a sterile solution of 0.1M acetic acid.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Hoeflich, A. et al. (1994) Hormone Research 41: 66
2. Gruslin, A. et al. (2005) The National Acad. of Sci.