Anti-Human IGF-II – Biotin

Anti-Human IGF-II – Biotin

Product No.: I-241

[product_table name="All Top" skus="I-241"]

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Product Type
Polyclonal Antibody
Alternate Names
Somatamedin A, Somatomedin C, C11orf43, FLJ22066, FLJ44734, INSIGF, pp9974, Insulin-Like Growth Factor II

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Antibody Details

Product Details

Reactive Species
Host Species
Purified Recombinant Human IGF-II (Accession # P01344)
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
State of Matter
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human IGF-II this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human IGF-II is 5 and 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-181.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.


Goat Anti-Human Insulin Like Growth Factor-II (IGF-II) recognizes Human IGF-II. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Insulin-like growth factor 2 (IGF-2) is a polypeptide hormone with structural and functional homology with IGF-I and pro-insulin.1 IGF-2 exerts its effects by binding to the IGF-1 receptor. IGF2 may also bind to the IGF-2 receptor. The major role of IGF2 is growth promoting hormone during gestation. IGF-2 has been shown to be an important regulator of fetoplacental growth.2

Antigen Details

NCBI Gene Bank ID

References & Citations

1. Hoeflich, A. et al. (1994) Hormone Research 41: 66
2. Gruslin, A. et al. (2005) Proc Natl Acad Sci U S A. 102(31):11047-52.
General Western Blot Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.