Recombinant Human IGFBP-1

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Pricing & Details

Product. No.I-370
Alternate Names
Insulin-Like Growth Factor Binding Protein 1, IBP-1, Placenta Protein 12 (PP12), AFBP, IGF-BP25, HIGFBP-1
Expression Host
NS0 cells
Species
Human
Prod No.
Size
Price
Avail.
Qty
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I-370-25 µg
25 µg
$349.00
In stock
Max:
Min: 1
Step: 1

Background

Insulin-like growth factor binding protein 1, also known as IGFBP1, is a member of the insulin-like growth factor binding protein (IGFBP) family that are cysteine-rich proteins that act as a carrier protein. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. IGFBP-1 is particularly important in female reproductive physiology, where it is involved with other factors in a complex system which regulates menstrual cycles, puberty, ovulation, decidualization, implantation and fetal growth. There is evidence for a pathophysiological role for IGFBP-1 in pre-eclampsia, intrauterine growth restriction, polycystic ovarian syndrome and trophoblast and endometrial neoplasms.1

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF‑7 human breast cancer cells.2 In the presence of 6 ng/mL recombinant human IGF-I, the ED50 for this effect is typically 0.1-0.6 µg/mL.
Expression Host
NS0 cells
Protein Accession No.
Amino Acid Sequence
apwqc apcsaeklal cppvsascse vtrsagcgcc pmcalplgaa cgvatarcar glscralpge qqplhaltrg qgacvqesda saphaaeags pespesteit eeelldnfhl mapseedhsi lwdaistydg skalhvtnik kwkepcriel yrvveslaka qetsgeeisk fylpncnkng fyhsrqcets mdgeaglcwc vypwngkrip gspeirgdpn cqiyfnvqn
N-terminal Sequence Analysis
Ala26
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human IGFBP-1 is Mr 25 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 32 kDa.
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank

References & Citations

1. Miell, JP. et al. (2000) Human Reproduction 6:495
2. Karey, KP. et al. (1988) Cancer Research 48:4083
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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