Insulin-like growth factor binding protein 1, also known as IGFBP1, is a member of the insulin-like growth factor binding protein (IGFBP) family that are cysteine-rich proteins that act as a carrier protein. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. IGFBP-1 is particularly important in female reproductive physiology, where it is involved with other factors in a complex system which regulates menstrual cycles, puberty, ovulation, decidualization, implantation and fetal growth. There is evidence for a pathophysiological role for IGFBP-1 in pre-eclampsia, intrauterine growth restriction, polycystic ovarian syndrome and trophoblast and endometrial neoplasms.1
>97% by SDS-PAGE and analyzed by silver stain.
<0.1 EU/µg as determined by the LAL method
Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF‑7 human breast cancer cells.2 In the presence of 6 ng/mL recombinant human IGF-I, the ED50 for this effect is typically 0.1-0.6 µg/mL.
Protein Accession No.
Amino Acid Sequence
apwqc apcsaeklal cppvsascse vtrsagcgcc pmcalplgaa cgvatarcar glscralpge qqplhaltrg qgacvqesda saphaaeags pespesteit eeelldnfhl mapseedhsi lwdaistydg skalhvtnik kwkepcriel yrvveslaka qetsgeeisk fylpncnkng fyhsrqcets mdgeaglcwc vypwngkrip gspeirgdpn cqiyfnvqn
N-terminal Sequence Analysis
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human IGFBP-1 is Mr 25 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 32 kDa.
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
Next Day Ambient
NCBI Gene Bank
References & Citations
1. Miell, JP. et al. (2000) Human Reproduction 6:495
2. Karey, KP. et al. (1988) Cancer Research 48:4083
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.