Recombinant Human IGFBP-5

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Pricing & Details

Product. No.I-374
Alternate Names
Insulin-Like Growth Factor Binding Protein 5, BP5
Expression Host
NS0 cells
Species
Human
Prod No.
Size
Price
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I-374-25 µg
25 µg
$349.00
In stock
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Min: 1
Step: 1

Background

Insulin-like growth factor binding protein 5, also known as IGFBP5 is a member of the insulin-like growth factor binding protein (IGFBP) family that are cysteine-rich proteins that act as a carrier protein. It is expressed highly in the kidney. IGFBP-5 has an autonomous role in the regulation of cell fate in development and in tumourigenesis.1

Protein Details

Purity
>90% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.01EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human IGFBP-5 was determined by by its ability to inhibit the biological activity of rhIGF-I or rhIGF-II on MCF-7 cells.2 In the presence of 14 ng/mL rhIGF-II, the expected ED50 for this effect is typically 0.5 - 1.5 μg/mL.
Expression Host
NS0 cells
Protein Accession No.
Amino Acid Sequence
epc dekalsmcpp splgcelvke pgcgccmtca laegqscgvy tercaqglrc lprqdeekpl hallhgrgvc lneksyreqv kierdsrehe epttsemaee tyspkifrpk htriselkae avkkdrrkkl tqskfvggae ntahpriisa pemrqeseqg pcrrhmeasl qelkasprmv pravylpncd rkgfykrkqc kpsrgrkrgi cwcvdkygmk lpgmeyvdgd fqchtfdssn ve
N-terminal Sequence Analysis
Glu28 & Met-Leu21
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human IGFBP-5 is Mr 28-29 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 35-37 kDa.
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank

References & Citations

1. Pell, JM. et al. (2004) J of Cell Science 117: 1737
2. Karey, KP. et al. (1988) Cancer Research 48:4083.
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Prod No.
Description
I-374
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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