Recombinant Human IL-1α

IL-1R1 is an 80 kD transmembrane glycoprotein and a member of the Ig superfamily. IL-1 binds both IL-1R1 and IL-1R2. The ligands IL-1α and IL-1β only commence when binding IL-1R1 because IL-1R2 acts as a decoy receptor and competitively antagonizes the binding of IL-1α and IL-1β, thereby reducing their biological effects. Binding of the ligands to IL-1R1 is enhanced by an accessory protein, IL-1R-AcP. IL-1α can exist as either a 31 kD precursor or an 18 kD mature form. IL-1α is a proinflammatory cell-associated cytokine and IL-1β is a 17.5 kD proinflammatory secreted cytokine. Both IL-1α and IL-1β bind to the same receptor and have similar properties that include stimulation of thymocyte proliferation via IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. Furthermore, IL-1β mediates a variety of immune and inflammatory responses. IL-1β is a major cause of severe inflammation in the mouse model of Crohn’s Disease, which makes IL-1β an important target for this disease.

The biological activity of Human IL-1 Alpha was determined in a cell proliferation assay using the murine helper T cell line, D10.G4.1 (Symons, J.A. et al., in Lymphokines and Interferons, a Practical Approach, 1987, M.J. Clemens, A.G. Morris, and A.J.H. Gearing, eds., IRL Press, p. 272). The expected ED₅₀ for this effect is typically 3 - 7 pg/ml.

Q53QF9

sapfsfls nvkynfmrii kyefilndal nqsiirandq yltaaalhnl deavkfdmga yksskddaki tvilrisktq lyvtaqdedq pvllkempei pktitgsetn llffwethgt knyftsvahp nlfiatkqdy wvclaggpps itdfqilenq a

Ser113

Solution

The predicted molecular weight of Recombinant Human IL-1α is Mr 18 kDa. Additionally, the actual molecular weight as observed by migration on SDS-PAGE is 18 kDa (reducing conditions).

18

This recombinant protein solution was 0.2 µm filtered and formulated in modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.

Working aliquots of human rIL-1 alpha are stable for up to twelve months at -20° C to -70° C in a manual defrost freezer. Upon thawing, rIL-1 alpha, in the presence of a carrier protein, can be stored at 2° - 8°C for one month without detectable loss of activity. For long-term storage, aliquot and freeze at -20° C to -70° C in a manual defrost freezer. Avoid repeated freeze thaw cycles.

Dry Ice

1. Arend, WP. et al. (2003) Cytokine Growth Factor Rev. 13:323
2. Nicklin, MJ. et al. (1994) Genomics 19:382
3. Dunn, E. et al. (2001) Trends Immunol. 22:533
4. Hardy, R. et al. (2009) J. Endocrinol. 201:309