Anti-Mouse IL-1α (Clone 40508)

Pricing & Details

Product No.I-658
Clone
40508
Protein
IL-1α
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Hematopoietin-1, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), Interleukin-1 Alpha
Isotype
Rat
IgG2b
Applications
ELISA Cap
,
N
Prod No.
Size
Price
Avail.
Qty
Add to cart
I-658-25 µg
25 µg
$225.00
In stock
Max:
Min: 1
Step: 1
I-658-100 µg
100 µg
$425.00
In stock
Max:
Min: 1
Step: 1
I-658-500 µg
500 µg
$925.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
Purified Recombinant Mouse IL-1α (>98%)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:I-245 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:I-204.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Mouse IL-1α bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse IL-1α (at 50 ng/ml) is 0.2 - 0.6 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 40508 recognizes an epitope on mouse IL-1α.
Background
IL-1R1 is an 80 kD transmembrane glycoprotein and a member of the Ig superfamily. IL-1 binds both IL-1R1 and IL-1R2. The ligands IL-1α and IL-1β only commence when binding IL-1R1 because IL-1R2 acts as a decoy receptor and competitively antagonizes the binding of IL-1α and IL-1β, thereby reducing their biological effects. Binding of the ligands to IL-1R1 is enhanced by an accessory protein, IL-1R-AcP. IL-1α can exist as either a 31 kD precursor or an 18 kD mature form. IL-1α is a proinflammatory cell-associated cytokine and IL-1β is a 17.5 kD proinflammatory secreted cytokine. Both IL-1α and IL-1β bind to the same receptor and have similar properties that include stimulation of thymocyte proliferation via IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. Furthermore, IL-1β mediates a variety of immune and inflammatory responses. IL-1β is a major cause of severe inflammation in the mouse model of Crohn’s Disease, which makes IL-1β an important target for this disease.

Antigen Details

Protein
PubMed

References & Citations

1.) Sasaki et. el; The Journal of Immunology July 17, 2013, 1202194 Journal Link
Elisa Sandwich Protocol

Formats Available

Prod No.
Description
I-658
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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