Recombinant Human IL-16

Interleukin 16 (lymphocyte chemoattractant factor), also known as IL16, is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. IL-16 is released by a variety of cells (including lymphocytes and some epithelial cells) that has been characterized as a chemoattractant for certain immune cells expressing the cell surface molecule CD4. IL-16 has a critical role in protecting antigen presenting cells (APC) against HIV-1 infection and is used in the treatment of HIV disease.¹

The biological activity of Human IL-16 (130 a.a.) has not been determined. The expected ED₅₀ in a biological assay has not been determined. However, human IL-16 may be used as a standard in an ELISA assay to create a standard curve.

The DNA sequence encoding the putative 130 aa residue IL-16 monomer was inserted into a suitable expression vector and was expressed in E. coli (Cruikshank, W.W. et al., 1994, Proc. Natl. Acad. Sci. USA 91:5109 - 5113).

Lyophilized

The predicted molecular weight of Recombinant Human IL-16 is Mr 14 kDa.

14

This recombinant protein solution was 0.2 µm filtered and formulated in modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.

Working aliquots of this recombinant protein solution are stable for up to twelve months at -20°C to -70°C in a manual defrost freezer. Upon thawing, in the presence of a carrier protein, this recombinant protein can be stored at 2° - 8°C for one month without detectable loss of activity. For long-term storage, aliquot and freeze at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles.

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1. Bahr GM. et al. (1999) J. Virol. 73: 7008