Recombinant Human IL-22

IL (interleukin)-22 is an IL-10 homologue, produced by activated T cells that binds to and signals via the class II cytokine receptor (R) heterodimer IL-22RA1/CFR2-4 (IL-10R2).¹ IL-22 regulates the production of acute phase proteins of the immunological response. On binding to its cognate receptor (IL-22R1), which is associated to the interleukin-10 receptor 2, IL-22 promotes activation of signal transducer and activator of transcription (STAT) pathway and several other cellular responses.² IL-22 plays an important role in skin inflammatory processes and wound healing.³

The biological activity of Human IL-22 was determined by its ability to induce IL-10 secretion in Colo205 cells (Nagalakshmi, M.L. et al., 2004, International Immunopharmacology. 4:679-691 The expected ED₅₀ for this effect is typically 60-300 pg/mL.

The DNA sequence encoding the mature human IL-22 protein sequence (Ala 34 - Ile 179) was inserted into a suitable expression vector and was expressed in E coli (Dumoutier, L. et al., 2000, PNAS 97:10144).

Lyophilized

The predicted molecular weight of Recombinant Human IL-22 is Mr 16.5 kDa.

16.5

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Yssel, H. et al.(2002) Int. Immunol. 14: 1351
2. Polikarpov, I. et al. (2006) Vitam Horm. 74: 77
3. Morel, F. et al. (2005) J. Immunol. 174: 3695