Recombinant Human IL-5

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Pricing & Details

Product. No.I-187
Alternate Names
Interleukin-5, EDF, BCDFII, TRF
Expression Host
Sf 21 Insect Cells
Species
Human
Prod No.
Size
Price
Avail.
Qty
Add to cart
I-187-5 µg
5 µg
$189.00
In stock
Max:
Min: 1
Step: 1
I-187-25 µg
25 µg
$569.00
In stock
Max:
Min: 1
Step: 1

Background

IL-5 is a 26 kD TH2 cytokine and a homodimeric glycoprotein that is part of the hematopoietic family. IL-5 stimulates B cell growth as well as increases Ig secretion. It is the main factor that promotes the terminal differentiation of eosinophil progenitors and enhances the effector capacity of mature eosinophils. IL-5 is a major regulator of eosinophil accumulation in tissues. In fact, in Hodgkin lymphoma, the characteristically observed eosinophilia is suspected to result from an increased production of IL-5. IL-5 may also be the cause of several allergic diseases including asthma, where it is thought that eosinophils have a significant role in the disease pathology. There are several mAbs currently in use that target IL-5 for the treatment of severe eosinophilic asthma.

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.01EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human IL-5 was determined in a cell proliferation assay using a factor-dependent human cell line, TF-1 (Kitamura, T. et al., 1989, J. Cell Physiol. 140:323 - 334). The expected ED50 for this effect is typically 0.1 - 0.2 ng/ml.
Expression Host
Sf 21 Insect Cells
Protein Accession No.
Amino Acid Sequence
i pteiptsalv ketlallsth rtllianetl ripvpvhknh qlcteeifqg igtlesqtvq ggtverlfkn lslikkyidg qkkkcgeerr rvnqfldylq eflgvmntew iies
N-terminal Sequence Analysis
Ile20
State of Matter
Solution
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human IL-5 is Mr 13.1 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 13-15 kDa.
Formulation
This recombinant protein solution was 0.2 µm filtered and formulated in modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.
Storage and Stability
Working aliquots of this recombinant protein solution are stable for up to twelve months at -20°C to -70°C in a manual defrost freezer. Upon thawing, in the presence of a carrier protein, this recombinant protein can be stored at 2° - 8°C for one month without detectable loss of activity. For long-term storage, aliquot and freeze at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
PubMed
NCBI Gene Bank

References & Citations

1. Capron, M. et al. (1994) J. Exp. Med. 179:703
2. Graber, P. et al. (1993) Nature 363:172
3. Inokuma, S. et al. (2005) Int. Arch. Allergy Immunol. 1:55
4. Shen, HH. et al. (2003) J. Immunol. 170:3296
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.