Macrophage migration inhibiting factor (MIF) is a pleiotropic protein that is the key mediator of the innate immune system and plays a crucial role in the host response to bacterial infections.1 MIF acts as a proinflammatory cytokine, a pituitary hormone, and a cell proliferation and migration factor.2 MIF plays a critical role in pathogenesis of ultraviolet-B (UVB)-induced nonmelanoma skin cancer (NMSC).3
>95% by SDS-PAGE and HPLC
<0.1 EU/µg as determined by the LAL method
The activity is determined by the dose-dependent induction of IL-8 from human PBMCs and is typically less than 50.0 ng/mL. This corresponds to an expected specific activity of 2 x 104 units/mg.
Protein Accession No.
Amino Acid Sequence
MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFA
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human MIF is Mr 12.5 kDa.
Lyophilized from a sterile filtered aqueous solution containing 10 mM Trisodium phosphate (Na3PO4), pH 7.5.
Storage and Stability
The lyophilized protein should be stored desiccated at -20°C - -70°C. The reconstituted protein can be stored for at least four (4) weeks at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day Ambient
NCBI Gene Bank
References & Citations
1. Pascal, RA. et al. (2005) Infec. Immun 73: 3783
2. Yamazaki, M. et al. (2009) Acta Neuropathol. 117: 321
3. Vanbuskirk, AM. et al. (2008) FASEB J.
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.