Recombinant Human MIF

Macrophage migration inhibiting factor (MIF) is a pleiotropic protein that is the key mediator of the innate immune system and plays a crucial role in the host response to bacterial infections.¹ MIF acts as a proinflammatory cytokine, a pituitary hormone, and a cell proliferation and migration factor.² MIF plays a critical role in pathogenesis of ultraviolet-B (UVB)-induced nonmelanoma skin cancer (NMSC).³

The activity is determined by the dose-dependent induction of IL-8 from human PBMCs and is typically less than 50.0 ng/mL. This corresponds to an expected specific activity of 2 x 104 units/mg.

MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFA

Lyophilized

The predicted molecular weight of Recombinant Human MIF is Mr 12.5 kDa.

12.5

Lyophilized from a sterile filtered aqueous solution containing 10 mM Trisodium phosphate (Na3PO4), pH 7.5.

The lyophilized protein should be stored desiccated at -20°C - -70°C. The reconstituted protein can be stored for at least four (4) weeks at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Next Day Ambient

1. Pascal, RA. et al. (2005) Infec. Immun 73: 3783
2. Yamazaki, M. et al. (2009) Acta Neuropathol. 117: 321
3. Vanbuskirk, AM. et al. (2008) FASEB J.