Recombinant Human MMP-13

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Pricing & Details

Product. No.M1242
Alternate Names
Collagenase 3, CLG3, CL3
Expression Host
NS0 cells
Species
Human
Prod No.
Size
Price
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M1242-10 µg
10 µg
$399.00
In stock
Max:
Min: 1
Step: 1

Background

Matrix metallopeptidase 13 (collagenase 3), also known as MMP13, is a glycosylated protein1 and a member of the matrix metalloproteinase (MMP) family of neutral endopeptidases, which is expressed in the skeleton during embryonic development2 and are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. MMP-13 is tightly bound to tissues and utilizes heparan sulfate proteoglycans as extracellular docking molecules. It is produced by many different cell types as an inactive proform that requires proteolytic activation to gain activity.3 Mutations causing lack of MMP-13 expression have been shown to be associated with skeletal disorders characterized by defective growth and modeling of the spine and long bones (spondyloepimetaphyseal dysplasias).4

Protein Details

Purity
>95% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human MMP-13 was determined by its ability to cleave the fluorogenic peptide substrate, MCAPLGLDPAARN H2. The specific activity is > 2,000 pmoles/min/μg.
Expression Host
NS0 cells
Protein Accession No.
Amino Acid Sequence
lplpsggded dlseedlqfa erylrsyyhp tnlagilken aassmterlr emqsffglev tgklddntld vmkkprcgvp dvgeynvfpr tlkwskmnlt yrivnytpdm thsevekafk kafkvwsdvt plnftrlhdg iadimisfgi kehgdfypfd gpsgllahaf ppgpnyggda hfdddetwts sskgynlflv aahefghslg ldhskdpgal mfpiytytgk shfmlpdddv qgiqslygpg dedpnpkhpk tpdkcdpsls ldaitslrge tmifkdrffw rlhpqqvdae lfltksfwpe lpnridaaye hpshdlifif rgrkfwalng ydilegypkk iselglpkev kkisaavhfe dtgktllfsg nqvwryddtn himdkdyprl ieedfpgigd kvdavyekng yiyffngpiq feysiwsnri vrvmpansil wc
N-terminal Sequence Analysis
Leu20
State of Matter
Solution
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human MMP-13 is Mr 52 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 58 kDa.
Formulation
The protein is supplied as a 0.2 µm filtered solution of 25 mM MES, 10 mM CaCl2, 0.15 M NaCl, 0.05% Brij-35, pH 6.0 containing 10 µg of rhMMP-13 at a concentration of 0.2 mg/ml. It is recommended to centrifuge the vial prior to opening.
Storage and Stability
This recombinant protein is stable for six months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Polar Packs
PubMed
NCBI Gene Bank

References & Citations

1. Knäuper, V. et al. (1996) J Biol. Chem. 271: 17124
2. Krane, SM. et al. (2004) Proc Natl Acad Sci U S A. 101(49):17192-7.
3. Woessner, JF. et al. (2000) J Biol. Chem. 275: 4183
4. Kennedy, AM. et al. (2005) J Clin. Invest. 115: 2832
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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