Anti-Human MMP-13 – Biotin

Anti-Human MMP-13 – Biotin

Product No.: M1309

[product_table name="All Top" skus="M1309"]

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Target
MMP-13
Product Type
Polyclonal Antibody
Alternate Names
Collagenase 3, CLG3, CL3
Applications
WB

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Product Size
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Goat
Immunogen
Purified Recombinant Human MMP-13 (Accession # P45452)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human MMP-13 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human MMP-13 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M1242.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Human Matrix Metalloproteinase 13 (MMP-13) recognizes Human MMP-13. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Matrix metallopeptidase 13 (collagenase 3), also known as MMP13, is a glycosylated protein1 and a member of the matrix metalloproteinase (MMP) family of neutral endopeptidases, which is expressed in the skeleton during embryonic development2 and are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. MMP-13 is tightly bound to tissues and utilizes heparan sulfate proteoglycans as extracellular docking molecules. It is produced by many different cell types as an inactive proform that requires proteolytic activation to gain activity.3 Mutations causing lack of MMP-13 expression have been shown to be associated with skeletal disorders characterized by defective growth and modeling of the spine and long bones (spondyloepimetaphyseal dysplasias).4

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Knäuper, V. et al. (1996) J Biol. Chem. 271: 17124
2. Krane, SM. et al. (2004) National Acad. Scien. 101(49):17192-7.
3. Woessner, JF. et al. (2000) J Biol. Chem. 275: 4183
4. Kennedy, AM. et al. (2005) J Clin. Invest. 115: 2832
General Western Blot Protocol
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.