Anti-Human MMP-13 (Clone M31.387)

a synthetic peptide corresponding to amino acids 131 to 140 of human MMP-13

This purified antibody is formulated with no carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl.

This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

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Anti-Human MMP-13 (clone M31.387) was produced in mice using a synthetic peptide corresponding to amino acids 131 to 140 of human MMP-13. This antibody recognizes the approximately 54-60 kDa latent human MMP-13.

Matrix metallopeptidase 13 (collagenase 3), also known as MMP13, is a glycosylated protein¹ and a member of the matrix metalloproteinase (MMP) family of neutral endopeptidases, which is expressed in the skeleton during embryonic development² and are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. MMP-13 is tightly bound to tissues and utilizes heparan sulfate proteoglycans as extracellular docking molecules. It is produced by many different cell types as an inactive proform that requires proteolytic activation to gain activity.³ Mutations causing lack of MMP-13 expression have been shown to be associated with skeletal disorders characterized by defective growth and modeling of the spine and long bones (spondyloepimetaphyseal dysplasias).⁴

1. Knäuper, V. et al. (1996) J Biol. Chem. 271: 17124
2. Krane, SM. et al. (2004) Proc Natl Acad Sci U S A. 101(49):17192-7
3. Woessner, JF. et al. (2000) J Biol. Chem. 275: 4183
4. Kennedy, AM. et al. (2005) J Clin. Invest. 115: 2832