Recombinant Human β-NGF

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Pricing & Details

Product. No.N116
Alternate Names
Beta-Nerve Growth Factor, Beta-NGF, HSAN5, MGC161426, MGC161428, NGFB
Expression Host
NS0 cells
Species
Human
Prod No.
Size
Price
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N116-100 µg
100 µg
$299.00
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Max:
Min: 1
Step: 1

Data

Background

Nerve growth factor beta (NGFβ/beta-NGF) is a secreted protein of the neurotropin family and is important for the differentiation and survival of neurons and dermal keratinocytes (1). NGFβ is found in the hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells and eosinophils. Glutamate, vitamin D3, IL-6, FGF basic, astrocyte specific IL-1, TNF alpha, PDGF and TGF beta up-regulate while GABAergic neuronal activity, glucocorticoids and Schwann cell-specific TGF beta down-regulate NFGβ. Signaling by NFGβ occurs through two receptors: TrKs and NGF receptor. It enhances the differentiation and survival of B lymphocytes (2) and also has a possible role in allergy and tissue repair (3).

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human β-NGF was determined by in a cell proliferation assay using a factor-dependent human erythroleukemic cell line, TF-1 (Kitamura, T. et al., 1989, J. Cell Physiol. 140:323-334). The ED50 for this effect is 0.8 - 1.5 ng/ml. β-NGF was also measured by its ability to support the survival and neurite outgrowth of cultured embryonic chick dorsal root ganglia (DRG). The expected ED50 0.5 - 1.0 ng/ml (Davies, A.M., 1989, “Neurotrophic Factor Bioassay Using Dissociated Neurons” in Nerve Growth Factors, R.A. Rush Ed., John Wiley and Sons, Ltd. pp. 95-109).
Expression Host
NS0 cells
Protein Accession No.
CAA36832
Amino Acid Sequence
ssshpifhrg efsvcdsvsv wvgdkttatd ikgkevmvlg evninnsvfk qyffetkcrd pnpvdsgcrg idskhwnsyc ttthtfvkal tmdgkqaawr firidtacvc vlsrkavrra
N-terminal Sequence Analysis
Ser122
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human β-NGF is Mr 13.5 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is 13 kDa.
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from a sterile solution of 0.1M acetic acid.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank

References & Citations

1. Schenck, K. et al. (2007) Eur. J. Oral Sci. 115:344
2. Otten, U. et al. (1989) Proc. Natl. Acad. Sci. (USA) 76:10059
3. Epplen, JT. et al. (2008) BMC Med. Genet. 9:107
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Prod No.
Description
N116
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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