Recombinant Human β-NGF
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BackgroundNerve growth factor beta (NGFβ/beta-NGF) is a secreted protein of the neurotropin family and is important for the differentiation and survival of neurons and dermal keratinocytes (1). NGFβ is found in the hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells and eosinophils. Glutamate, vitamin D3, IL-6, FGF basic, astrocyte specific IL-1, TNF alpha, PDGF and TGF beta up-regulate while GABAergic neuronal activity, glucocorticoids and Schwann cell-specific TGF beta down-regulate NFGβ. Signaling by NFGβ occurs through two receptors: TrKs and NGF receptor. It enhances the differentiation and survival of B lymphocytes (2) and also has a possible role in allergy and tissue repair (3). Protein DetailsPurity >97% by SDS-PAGE and analyzed by silver stain. Endotoxin Level <0.1 EU/µg as determined by the LAL method Biological Activity The biological activity of Human β-NGF was determined by in a cell proliferation assay using a factor-dependent human erythroleukemic cell line, TF-1 (Kitamura, T. et al., 1989, J. Cell Physiol. 140:323-334). The ED50 for this effect is 0.8 - 1.5 ng/ml. β-NGF was also measured by its ability to support the survival and neurite outgrowth of cultured embryonic chick dorsal root ganglia (DRG). The expected ED<sub>50</sub> 0.5 - 1.0 ng/ml (Davies, A.M., 1989, “Neurotrophic Factor Bioassay Using Dissociated Neurons” in Nerve Growth Factors, R.A. Rush Ed., John Wiley and Sons, Ltd. pp. 95-109). Protein Accession No. CAA36832 Amino Acid Sequence ssshpifhrg efsvcdsvsv wvgdkttatd ikgkevmvlg evninnsvfk qyffetkcrd pnpvdsgcrg idskhwnsyc ttthtfvkal tmdgkqaawr firidtacvc vlsrkavrra
N-terminal Sequence Analysis Ser122 State of Matter Lyophilized Predicted Molecular Mass The predicted molecular weight of Recombinant Human β-NGF is Mr 13.5 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is 13 kDa. Predicted Molecular Mass 13.5 Formulation This recombinant protein was 0.2 µm filtered and lyophilized from a sterile solution of 0.1M acetic acid. Storage and Stability This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. Country of Origin USA Shipping Next Day Ambient NCBI Gene Bank References & Citations1. Schenck, K. et al. (2007) Eur. J. Oral Sci. 115:344 2. Otten, U. et al. (1989) Proc. Natl. Acad. Sci. (USA) 76:10059 3. Epplen, JT. et al. (2008) BMC Med. Genet. 9:107 IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein. |
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