Recombinant Human P-Selectin

Recombinant Human P-Selectin

Product No.: P302

[product_table name="All Top" skus="P302"]

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Product Type
Recombinant Protein
Expression Host
CHO Cells

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Protein Details

>97% by SDS Page and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human P-Selectin was determined by its ability to immobilize protein to support the adhesion of U937 human histiocytic lymphoma cells.
Protein Accession No.
Amino Acid Sequence
wtyhystkay swnisrkycq nrytdlvaiq nkneidylnk vlpyyssyyw igirknnktw twvgtkkalt neaenwadne pnnkrnnedc veiyikspsa pgkwndehcl kkkhalcyta scqdmscskq gecletigny tcscypgfyg peceyvrecg elelpqhvlm ncshplgnfs fnsqcsfhct dgyqvngpsk leclasgiwt nkppqclaaq cpplkiperg nmtclhsaka fqhqsscsfs ceegfalvgp evvqctasgv wtapapvcka vqcqhleaps egtmdcvhpl tafaygssck fecqpgyrvr gldmlrcids ghwsaplptc eaisceples pvhgsmdcsp slrafqydtn csfrcaegfm lrgadivrcd nlgqwtapap vcqalqcqdl pvpnearvnc shpfgafryq svcsftcneg lllvgasvlq clatgnwnsv ppecqaipct pllspqngtm tcvqplgsss ykstcqficd egyslsgper ldctrsgrwt dsppmceaik cpelfapeqg sldcsdtrge fnvgstchfs cdngfklegp nnvecttsgr wsatpptckg iaslptpglq cpalttpgqg tmycrhhpgt fgfnttcyfg cnagftligd stlscrpsgq wtavtpacra vkcselhvnk piamncsnlw gnfsygsics fhclegqlln gsaqtacqen ghwsttvptc qagpltiqea
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human GMP-140, LECAM-3, PADGEM, CD62P is Mr 80 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 121-124 kDa, reducing conditions kDa.
Predicted Molecular Mass
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
Next Day Ambient
NCBI Gene Bank
Applications and Recommended Usage ?
(Quality Tested by Leinco)
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.
Flow Cytometry: PN:A106
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CXCR5. A suggested method would be to stain cells expressing CXCR5 with approximately 10 µl per test. A typical test sample constitutes approximately 50 µl of packed whole blood or 1 x 105 continuous passage or activated cell cultures that have been centrifuged at 500 X g for five minutes. Labeling of the cells with the biotin conjugate should be followed by PN:A104, resuspended in 200-400 µl of 1X PBS.
Elisa Sandwich Protocol
Flow Cytometry
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.