Recombinant Human PDGF-AA

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Pricing & Details

Product. No.P125
Alternate Names
Glioma-Derived Growth Factor [GDGF], Osteosarcoma-Derived Growth Factor [ODGF]
Expression Host
E. coli
Species
Human
Prod No.
Size
Price
Avail.
Qty
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P125-10 µg
10 µg
$269.00
In stock
Max:
Min: 1
Step: 1
P125-25 µg
25 µg
$429.00
In stock
Max:
Min: 1
Step: 1
P125-50 µg
50 µg
$839.00
In stock
Max:
Min: 1
Step: 1

Background

Platelet-derived growth factor-BB homodimers (PDGF-BB), also known as GDGF and ODGF, is a member of the PDGF family. PDGFs are disulfide-linked dimers consisting of two polypeptide chains, designated PDGF-A and PDGF-B. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. They are stored in platelet α-granules and are released upon platelet activation. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β.1 PDGFR-α is high-affinity receptor for each of the three PDGF forms, while PDGFR-β interacts with only PDGF-BB and PDGF-AB. PDGF functions as a local autocrine and paracrine growth factor.2 PDGF-AA is widely expressed in normal and transformed cells and is a potent mitogen for a wide range of cell types including fibroblast, smooth muscle and connective tissue.3 The PDGF-AA/receptor complex represents an important epithelial-mesenchymal interaction which plays a critical role in early lung branching morphogenesis.4 It is also an essential autocrine regulator for vascular endothelial growth factor (VEGF) expression, regulating its frequency and level during the transition from a precancerous lesion to advanced cancer.5

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.01 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human PDGF-AA was determined by its ability to stimulate proliferation of NR6R-3T3 fibroblasts (Raines, E.W. et al., 1985, Methods Enzym. 109:749). The expected ED50 for this effect is typically 3 - 5 ng/ml when measured by 3H-thymidine incorporation.
Expression Host
E. coli
Protein Accession No.
Amino Acid Sequence
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N-terminal Sequence Analysis
Ser87 and Met
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human PDGF-AA is Mr 29 kDa.
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank

References & Citations

1. Pfeilschifter, J. et al. (2005) Am. Soc. Nephrol. 10:1681
2. Claesson-Welsh, L. et al. (1991) J. Biol. Chem. 266:21138
3. Deuel, TF. et al. (1992) Biochem. Biophys. Res. Commun. 186:1510
4. Post, M. et al. (1995) Endocrin. 121:25529
5. Sueishi, K. et al. (2005) Cancer Res. 65:7241
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.