Recombinant Human PDGF-AA

Platelet-derived growth factor-BB homodimers (PDGF-BB), also known as GDGF and ODGF, is a member of the PDGF family. PDGFs are disulfide-linked dimers consisting of two polypeptide chains, designated PDGF-A and PDGF-B. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. They are stored in platelet α-granules and are released upon platelet activation. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β.¹ PDGFR-α is high-affinity receptor for each of the three PDGF forms, while PDGFR-β interacts with only PDGF-BB and PDGF-AB. PDGF functions as a local autocrine and paracrine growth factor.² PDGF-AA is widely expressed in normal and transformed cells and is a potent mitogen for a wide range of cell types including fibroblast, smooth muscle and connective tissue.³ The PDGF-AA/receptor complex represents an important epithelial-mesenchymal interaction which plays a critical role in early lung branching morphogenesis.⁴ It is also an essential autocrine regulator for vascular endothelial growth factor (VEGF) expression, regulating its frequency and level during the transition from a precancerous lesion to advanced cancer.⁵

The biological activity of Human PDGF-AA was determined by its ability to stimulate proliferation of NR6R-3T3 fibroblasts (Raines, E.W. et al., 1985, Methods Enzym. 109:749). The expected ED₅₀ for this effect is typically 3 - 5 ng/ml when measured by 3H-thymidine incorporation.

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Ser87 and Met

Lyophilized

The predicted molecular weight of Recombinant Human PDGF-AA is Mr 29 kDa.

29

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Pfeilschifter, J. et al. (2005) Am. Soc. Nephrol. 10:1681
2. Claesson-Welsh, L. et al. (1991) J. Biol. Chem. 266:21138
3. Deuel, TF. et al. (1992) Biochem. Biophys. Res. Commun. 186:1510
4. Post, M. et al. (1995) Endocrin. 121:25529
5. Sueishi, K. et al. (2005) Cancer Res. 65:7241