Anti-Human PDGF-AA (Clone 114506)

Anti-Human PDGF-AA (Clone 114506)

Product No.: P274

[product_table name="All Top" skus="P274"]

- -
- -
Clone
114506
Target
PDGF-AA
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Glioma-Derived Growth Factor [GDGF], Osteosarcoma-Derived Growth Factor [ODGF]
Isotype
IgG1
Applications
N

- -
- -
Product Size
- -
- -

Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human PDGF-AA (Accession # P04085)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Additional Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human PDGF-AA bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human PDGF-AA (at 25 ng/ml) is 0.2 - 1.0 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human Platelet-Derived Growth Factor AA (PDGF-AA) (Clone 114506) recognizes an epitope on Human PDGF-AA. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows approximately 20% cross-reactivity with rhPDGF-AB and no cross-reactivity with rhPDGF-BB, rhPlGF, rhVEGF206, rhVEGF B186, rhVEGF C, rhVEGF D, or rhCTGF.
Background
Platelet-derived growth factor (PDGF), an agent with important mitogenic effects for bone cells, exists in three isoforms, PDGF-AA, - BB, and –AB.1 The A-chain homodimers of the platelet-derived growth factor (PDGF AA) are widely expressed in normal and transformed cells.2 PDGF AA is a potent mitogen for a wide range of cell types including fibroblast, smooth muscle, and connective tissue. PDGF-AA and its receptor represent an important epithelial-mesenchymal interaction which plays a critical role in early lung branching morphogenesis.3 PDGF AA is also an essential autocrine regulator for vascular endothelial growth factor (VEGF) expression. It regulates the frequency and level of VEGF expression during the transition from a precancerous lesion to advanced cancer.4

Antigen Details

PubMed

References & Citations

1. Canalis, E. et al. (1994) Endocrin. 134: 2541
2. Deuel, TF. et al. (1992) Biochem Biophys Res Commun. 186: 1510
3. Post, M. et al. (1995) Endocrin. 121: 25529
4. Sueishi, K. et al. (2005) Cancer Research 65: 7241
- -
- -

Formats Available

- -
- -
Prod No.
Description
P274
Products are for research use only. Not for use in diagnostic or therapeutic procedures.