Recombinant Human Prolactin

Prolactin (PRL) or Luteotropic hormone (LTH) is best known as the pituitary modulator of lactation and reproduction.¹ Prolactin is a multifaceted hormone that is capable of modulating hundreds of physiological processes in adult vertebrates.² PRL promotes proliferation, survival and migration of cancer cells acting via the prolactin receptor (PRLR).³ It also modulates maternal behavior and mediates hypothalamic pituitary adrenal axis inhibition during lactation via PRL receptors in the brain.⁴ Prolactin also has a number of other effects including contributing to surfactant synthesis of the fetal lungs at the end of the pregnancy and immune tolerance of the fetus by the maternal organism during pregnancy. It also decreases normal levels of sex hormones — estrogen in women and testosterone in men.⁵

The biological activity of Human PRL was measured in a cell proliferation assay using rat lymphoma cells, Nb2-11.⁶ The expected ED₅₀ for this effect is typically 0.03 - 0.1 ng/mL.

lp icpggaarcq vtlrdlfdra vvlshyihnl ssemfsefdk rythgrgfit kainschtss latpedkeqa qqmnqkdfls livsilrswn eplyhlvtev rgmqeapeai lskaveieeq tkrllegmel ivsqvhpetk eneiypvwsg lpslqmadee srlsayynll hclrrdshki dnylkllkcr iihnnnc

Met

Lyophilized

The predicted molecular weight of Recombinant Human PRL is Mr 24 kDa.

24

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Paus, R. et al. (2012) Arch Dermatol Res. 304(2):115-8.
2. Zhu, Y. et al. (2008) Comp Biochem Physiol C Toxicol Pharmacol. 148(4):370-80.
3. Panina, S. et al. (2009) J Endocrinol. 201(1):115-28.
4. Neuwmann, ID. et al. (2009) Endocrinology. 150(4):1841-9.
5. Molitch MD., ME. (2005) Mayo Clinic Proceedings 80(8):1050-1057
6. Gout, PW. et al. (1980) Cancer Research 40:2433-36.