Anti-Human Prolactin – Biotin

Anti-Human Prolactin – Biotin

Product No.: P294

[product_table name="All Top" skus="P294"]

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Target
Prolactin
Product Type
Polyclonal Antibody
Alternate Names
Mammotropin, Luterotropic Hormone (LTH), Lutetropin
Applications
ELISA Det
,
WB

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Product Size
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Goat
Immunogen
Purified Recombinant Human PRL (Accession # Q5THQ0)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human PRL this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human PRL is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:P168.
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml when used in conjunction with PN:P283 as the capture antibody at 0.2-0.8 µg/ml and an optimal second step reagent such as PN:A106 for the detection of Human PRL.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Human Prolactin (PRL) recognizes Human PRL. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Prolactin (PRL) or Luteotropic hormone (LTH) is best known as the pituitary modulator of lactation and reproduction.1 Prolactin is a multifaceted hormone that is capable of modulating hundreds of physiological processes in adult vertebrates.2 PRL promotes proliferation, survival and migration of cancer cells acting via the prolactin receptor (PRLR).3 It also modulates maternal behavior and mediates hypothalamic pituitary adrenal axis inhibition during lactation via PRL receptors in the brain.4 Prolactin also has a number of other effects including contributing to surfactant synthesis of the fetal lungs at the end of the pregnancy and immune tolerance of the fetus by the maternal organism during pregnancy. It also decreases normal levels of sex hormones — estrogen in women and testosterone in men.5

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Paus, R. et al. (2012) Arch Dermatol Res. 304(2):115-8.
2. Zhu, Y. et al. (2008) Comp Biochem Physiol C Toxicol Pharmacol. 148(4):370-80.
3. Panina, S. et al. (2009) J Endocrinol. 201(1):115-28.
4. Neuwmann, ID. et al. (2009) Endocrinology. 150(4):1841-9.
5. Molitch MD., ME. (2005) Mayo Clinic Proceedings 80(8):1050-1057
6. Gout, PW. et al. (1980) Cancer Research 40:2433-36.
ELISA Det
General Western Blot Protocol
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.