RANKL (Receptor Activator for Nuclear Factor κ B Ligand), also known as TNF-related activation-induced cytokine (TRANCE), osteoprotegerin ligand (OPGL), and ODF (osteoclast differentiation factor), is a member of the tumor necrosis factor family cytokine.1 RANKL is essential for osteoclast formation, activation, and survival.2 RANKL through interaction with its receptor RANK can modulate matrix degradation and inflammation.3 RANKL and RANK have essential roles in lymph node formation, establishment of the thymic microenvironment, and development of a lactating mammary gland during pregnancy. Consequently, novel drugs specifically targeting RANK, RANKL, and their signaling pathways in osteoclasts are expected to revolutionize the treatment of various ailments associated with bone loss, such as arthritis, periodontal disease, cancer metastases, and osteoporosis.4
>90% by SDS-PAGE and analyzed by silver stain.
<0.1 EU/µg as determined by the LAL method
The biological activity of Human sRANKL was determined by its ability to induce osteoclast differentiation on mouse splenocytes. The expected ED50= 5-15 ng/ml in the presence of 2.5 µg/ml of the cross-linking antibody, PN:P246.
Fusion Protein Tag
Protein Accession No.
Amino Acid Sequence
mhhhhhhhhh hgggsgggsg ggsiegrgsq hiraekamvd gswldlakrs kleaqpfahl tinatdipsg shkvslsswy hdrgwgkisn mtfsngkliv nqdgfyylya nicfrhhets gdlateylql mvyvtktsik ipsshtlmkg gstkywsgns efhfysinvg gffklrsgee isievsnpsl ldpdqdatyf gafkvrdid
N-terminal Sequence Analysis
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human sRANKL is Mr 23 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 35 kDa.
This recombinant protein was 0.2 µm filtered and lyophilized from 20 mM MOPS and 500 mM NaCL, pH 6.5 with 5% trehalose.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
Next Day Ambient
NCBI Gene Bank
References & Citations
1. Takayanagi, H. et al. (2003) Arthritis Res Ther. 5: 20
2. Kostenuik, PJ. et al. (2005) Endocrinology 146: 3235
3. Holven ,KB. et al. (2009) Stroke 40: 241
4. Penninger, JM. et al. (2008) Ann N Y Acad Sci. 1143: 123
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.