Anti-Mouse RANKL

Purified Recombinant Mouse RANKL (>98%)

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Lyophilized

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Next Day Ambient

Immunohistochemistry on Frozen Sections: This antibody should give satisfactory staining results when used on frozen sections at a concentration of 5-15 µg/ml.
Neutralization: The Neutralization Dose (ND₅₀) is typically 0.02-0.06 µg/mL in the presence of 30 ng/mL Recombinant Mouse TRANCE/TNFSF11/RANK L.

Rabbit Anti-Mouse soluble Receptor Activator of NFkB Ligand (RANKL) recognizes Mouse RANKL. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

RANKL (Receptor Activator for Nuclear Factor κ B Ligand), also known as TNF-related activation-induced cytokine (TRANCE), osteoprotegerin ligand (OPGL), and ODF (osteoclast differentiation factor), is a member of the tumor necrosis factor family cytokine.¹ RANKL is essential for osteoclast formation, activation, and survival.² RANKL through interaction with its receptor RANK can modulate matrix degradation and inflammation.³ RANKL and RANK have essential roles in lymph node formation, establishment of the thymic microenvironment, and development of a lactating mammary gland during pregnancy. Consequently, novel drugs specifically targeting RANK, RANKL, and their signaling pathways in osteoclasts are expected to revolutionize the treatment of various ailments associated with bone loss, such as arthritis, periodontal disease, cancer metastases, and osteoporosis.⁴

1. Takayanagi, H. et al. (2003) Arthritis Res Ther. 5: 20
2. Kostenuik, PJ. et al. (2005) Endocrinology 146: 3235
3. Holven, KB. et al. (2009) Stroke 40: 241
4. Penninger, JM. et al. (2008) Ann N Y Acad Sci. 1143: 123