Recombinant Mouse RANK Ligand

Recombinant Mouse RANK Ligand

Product No.: R1255

[product_table name="All Top" skus="R1255"]

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Alternate Names
CD254, TRANCE, hRANKL2, sOdf, TNF-Related Activation-Induced Cytokine [TRANCE], Osteoprotegrin Ligand [OPGL], Osteoclast Differentiation Factor [ODF], TNFSF11
Product Type
Recombinant Protein
Expression Host
NS0 Cells
Species
Mouse

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Product Size
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Background

RANKL (Receptor Activator for Nuclear Factor κ B Ligand), also known as TNF-related activation-induced cytokine (TRANCE), osteoprotegerin ligand (OPGL), and ODF (osteoclast differentiation factor), is a member of the tumor necrosis factor family cytokine.1 RANKL is essential for osteoclast formation, activation, and survival.2 RANKL through interaction with its receptor RANK can modulate matrix degradation and inflammation.3 RANKL and RANK have essential roles in lymph node formation, establishment of the thymic microenvironment, and development of a lactating mammary gland during pregnancy. Consequently, novel drugs specifically targeting RANK, RANKL, and their signaling pathways in osteoclasts are expected to revolutionize the treatment of various ailments associated with bone loss, such as arthritis, periodontal disease, cancer metastases, and osteoporosis.4

Protein Details

Purity
>95% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse RANKL was determined by its ability to induce osteoclast differentiation on mouse splenocytes. The expected ED<sub>50</sub> for this effect is typically 5-15 ng/ml in the presence of 2.5 μg/ml of a cross-linking antibody PN:P246.
Fusion Protein Tag
Histidine Tag
Protein Accession No.
Amino Acid Sequence
aqmdpnri sedsthcfyr ilrlhenadl qdstlesedt lpdscrrmkq afqgavqkel qhivgpqrfs gapammegsw ldvaqrgkpe aqpfahltin aasipsgshk vtlsswyhdr gwakisnmtl sngklrvnqd gfyylyanic frhhetsgsv ptdylqlmvy vvktsikips shnlmkggst knwsgnsefh fysinvggff klrageeisi qvsnpslldp dqdatyfgaf kvqdidhhhh hh
N-terminal Sequence Analysis
His
State of Matter
Solution
Predicted Molecular Mass
The predicted molecular weight of Recombinant Mouse RANKL is Mr 28 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 36 kDa.
Predicted Molecular Mass
28
Formulation
This recombinant protein solution was 0.2 µm filtered and formulated in modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.
Storage and Stability
This recombinant protein solution is stable for up to twelve months when stored at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. Upon thawing, this protein, in the presence of a carrier protein, it can be stored at 2° - 8° C for one month without detectable loss of activity. For long-term storage, aliquot and freeze at -20° C to -70° C in a manual defrost freezer. Avoid repeated freeze-thaw cycles..
Country of Origin
USA
Shipping
Dry Ice
NCBI Gene Bank

References & Citations

1. Takayanagi, H. et al. (2003) Arthritis Res Ther. 5: 20
2. Kostenuik, PJ. et al. (2005) Endocrinology 146: 3235
3. Holven, KB. et al. (2009) Stroke 40: 241
4. Penninger, JM. et al. (2008) Ann N Y Acad Sci. 1143: 123
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.