Recombinant Human TRAIL R1

Tumor necrosis factor-related apoptosis-inducing ligand Receptor 1 (TRAIL-R1) is a cell-surface receptor involved in tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced cell-death signaling.¹ The death ligand TRAIL bears high potential as a new anticancer agent, as binding to the death receptors TRAIL-R1 or TRAIL-R2 triggers apoptosis in most cancer cells.²

The biological activity of Human TNF-Related Apoptosis Inducing Ligand Receptor-1 is determined by its inhibitory effect of IL-8 production in human PBMC by human TRAIL/Apo2L. 95% inhibition (100 ng/ml of TRAIL/Apo2L) was reached using a concentration of 100 ng/ml of TRAIL Receptor-1.

Fc Fusion Protein

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Ala109 & Thr110

Lyophilized

The predicted molecular weight of Recombinant Human TRAIL R-1 is Mr 43 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 46 kDa.

43

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Yoo, NJ. et al. (2001) Oncogene 20: 399
2. Eberle, J. et al. (2005) J Investigative Dermatol. 125: 1010
3. Rimm, D. et al. (2005) Clin Cancer Research 11