Recombinant Human TGF-β1 (Insect Cells)

Recombinant Human TGF-β1 (Insect Cells)

Product No.: L193

[product_table name="All Top" skus="L193"]

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Prod. No.L193
Expression Host
sf Insect Cells

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Background

Transforming growth factor-beta 1 (TGF-β1), also known as LAP, is a member of the TGF beta family of growth factors along with three other TGF-β isoforms; TGF-β2 and -3. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix (1). They are involved in various physiological processes including embryogenesis, tissue remodeling, wound healing, osteogenesis and immune cell function (1). The three isoforms are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-β from a latent complex involves proteolytic processing of the complex and/or induction of conformational changes, by proteins such as thrombospondin-1 or MMP (2). TGF-β1 is the most abundant isoform secreted by almost every cell type. Regulatory T cells release TGF-β1 to inhibit the actions of other T cells (3). Similarly, TGF-β1 can inhibit the secretion and activity of many other cytokines including interferon-γ, TNF-α and various interleukins. It can also decrease the expression levels of cytokine receptors, such as the IL-2 receptor, to down-regulate the activity of immune cells. TGF-β1 has similar effects on B cells that also vary according to the differentiation state of the cell (4). It inhibits proliferation and apoptosis of B cells, and plays a role in controlling the expression of antibody, transferrin and MHC class II proteins on immature and mature B cells.

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human LAP was determined by its ability to inhibit TGF-β1 activity on mouse HT-2 cells. The ED<sub>50</sub> for this effect is typically 50 - 300 ng/ml in the presence of 1 ng/ml of TGF-β1.
Amino Acid Sequence
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State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human LAP is Mr 27 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is 28-36 kDa, reducing and 60-70 kDa, non-reducing conditions (variably glycosylated).
Predicted Molecular Mass
27
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Sporn, MB. et al. (2006) Cytokine Growth factor Rev. 17:3
2. Oklu, R. et al. (2000) Biochem. J. 352:601
3. Wahl, S. et al. (1988) J. Immunol. 140:3026
4. Lebman, D. et al. (1999) Microbes Infect. 1:1297
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.