Transforming growth factor beta 1 or TGF-β1 is a multifunctional cytokine that regulates the proliferation and differentiation of cells specific to bone like chondrocytes, osteoblasts, osteoclasts including mesenchymal precursor cells.1 It is known to promote the pathogenesis of lung fibrosis and acts as a tumor suppressor in normal cells.2 TGF-beta1 can promote mechanical strength in healing Achilles tendons by regulating collagen synthesis, cross-link formation, and matrix remodeling.3
>97% by SDS-PAGE and analyzed by silver stain.
<0.1 EU/µg as determined by the LAL method
The biological activity of Human Transforming Growth Factor-Beta 1 (mammalian derived) is determined by its ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells. The expected ED50 for this effect is 0.04 - 0.2 ng/ml.
Protein Accession No.
Amino Acid Sequence
The DNA sequence encoding the human TGF-β1 precursor protein (Derynck, R. et al., 1985, Nature 316:701 - 705) was inserted into a suitable mammalian expression vector and expressed in CHO cells.
N-terminal Sequence Analysis
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human TGF-β1 is Mr 12.8 kDa. However, the actual molecular weight as observed by migration on SDS Page is 12 kDa (reducing conditions) and 24 kDa (non-reducing conditions)
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
Next Day Ambient
NCBI Gene Bank
References & Citations
1. Guven, M. et al. (2007) Growth Factors 25: 101
2. Schneider, J. et al. (2009) J Investing Med. 57(5):655-61
3. Yu, C. et al. (2009) Biochem Biophys Res Commun. 406(2):204-210
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.