Anti-Human TGF-β1 (Clone 27235)

Purified Recombinant Human TGF-β1 (>98%)

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Next Day Ambient

Neutralization: This antibody is useful for neutralization of Human LAP (TGF-β1) bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human LAP (TGF-β1) (at 125 ng/ml) is 0.5-1.0 µg/ml.
Immunocytochemistry: Suitable for use at concentration of 3-25 µg/mL.

Mouse Anti-Human Latency Associated Peptide (LAP) (Clone 27235) recognizes an epitope on Human LAP. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Transforming growth factor beta 1 or TGF-β1 is a multifunctional cytokine that regulates the proliferation and differentiation of cells specific to bone like chondrocytes, osteoblasts, osteoclasts including mesenchymal precursor cells.¹ It is known to promote the pathogenesis of lung fibrosis and acts as a tumor suppressor in normal cells.² TGF-beta1 can promote mechanical strength in healing Achilles tendons by regulating collagen synthesis, cross-link formation, and matrix remodeling.³

1. Guven, M. et al. (2007) Growth Factors 25: 101
2. Schneider, J. et al. (2009) J Investing Med. 57(5):655-61
3. Yu, C. et al. (2009) Biochem Biophys Res Commun. 406(2):204-210