Recombinant Human XEDAR

XEDAR (X-linked Ectodysplasin A2 Receptor) is a type II transmembrane and member of the TNF receptor superfamily. It is highly expressed during embryonic development and induces apoptosis (1). XEDAR is a receptor for Ectodysplasin-A2 (EDA-A2) which may be a promising agent for the gene therapy of osteosarcoma (2). It has also been associated with hypohidrotic ectodermal dysplasia (HED).

The biological activity of Human XEDAR was determined by its ability to bind rhEDA-A2/His. Immobilized rhXEDAR/Fc at 1 μg/mL (100 μL/well) can bind rhEDA-A2/His with a linear range of 0.045 - 5 ng/mL in a functional ELISA.

Fc Fusion Protein

mdcqeneywd qwgrcvtcqr cgpgqelskd cgygeggday ctacpprryk sswghhrcqs citcavinrv qkvnctatsn avcgdclprf yrktrigglq dqecipctkq tptsevqcaf qlslveadap tvppqedieg rmdpkscdkt htcppcpape llggpsvflf ppkpkdtlmi srtpevtcvv vdvshedpev kfnwyvdgve vhnaktkpre eqynstyrvv svltvlhqdw lngkeykckv snkalpapie ktiskakgqp repqvytlpp srdeltknqv sltclvkgfy psdiavewes ngqpennykt tppvldsdgs fflyskltvd ksrwqqgnvf scsvmhealh nhytqkslsl spgk

Met1

Lyophilized

The predicted molecular weight of Recombinant Human XEDAR is Mr 41.8 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 61 kDa.

41.8

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Preet, M. et al. (2004) J. Biol. Chem. 279:41873
2. Chaudhary, PM. et al. (2007) Cancer Gene Ther. 14:927