Recombinant Mouse EphB4

The biological activity of Mouse EphB4 was determined by its binding ability in a functional ELISA. Immobilized recombinant mouse EphB4 at 2 μg/ml (100 μl/well) can bind recombinant mouse EphrinB2 with a linear range of 0.078-5 ng/ml.

Fc Fusion Protein

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Leu16

Lyophilized

The predicted molecular weight of Recombinant Mouse EphB4 is Mr 85.2 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 110 kDa.

85.2

This recombinant protein was 0.2 µm filtered and lyophilized from a sterile solution containing 10mM Tris, 75 mM NaCl pH 7.5.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Wu, J. et al. (2015) J Biol Chem. 290(22):14235-44
2. Gronthos, S. et al. (2015) Stem Cells. 33(9):2838-49.