Fibroblast growth factor 9 (glia-activating factor), also known as FGF9 is a glycosylated neurotrophic polypeptide highly expressed in brain.1 It is a member of the fibroblast growth factor (FGF) family that possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. FGF-9 has sequence similarity of approximately 30% to other members of the family of fibroblast growth factors.2 It is highly related to FGF-16. FGF-9 has been shown to mediate its effects by binding to FGF receptors. It efficiently activates the FGFR2c splice form of FGFR2 and the FGFR3b and FGFR3c splice isoforms of FGFR3.3 FGF-9 is a high affinity, heparin dependent ligand for FGFR3 and FGFR2 but not for FGFR1 and FGFR4.4
>95% by SDS Page and HPLC
The biological activity of Mouse Fibroblast Growth Factor-9 is determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors. The expected ED50 for this effect is 2 x 106 units/mg.
Protein Accession No.
Amino Acid Sequence
PLGEVGSYFG VQDAVPFGNV PVLPVDSPVL LNDHLGQSEA GGLPRGPAVT DLDHLKGILR RRQLYCRTGF HLEIFPNGTI QGTRKDHSRF GILEFISIAV GLVSIRGVDS GLYLGMNEKG ELYGSEKLTQ ECVFREQFEE NWYNTYSSNL YKHVDTGRRY YVALNKDGTP REGTRTKRHQ KFTHFLPRPV DPDKVPELYK DILSQS
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Mouse FGF-9 is Mr 23 kda kDa.
Storage and Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day Ambient
References & Citations
1. Imamura T. et al. (1999)J Biol Chem 274: 29352 2. Miyamoto M et al. (1993)Molecular Cellular Biology 13: 4251 3. Santos-Ocampo S et al. (1996)Journal of Biological Chemistry 271: 1726 4. Hecht D et al. (1995)Growth Factors 12: 223
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.